Physical and chemical characteristics of apolipoprotein A-I-lipid complexes produced by Chinese hamster ovary cells transfected with the human apolipoprotein A-I gene》.pdfVIP

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Physical and chemical characteristics of apolipoprotein A-I-lipid complexes produced by Chinese hamster ovary cells transfected with the human apolipoprotein A-I gene》.pdf

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Physical and chemical characteristics of apolipoprotein A-I-lipid complexes produced by Chinese hamster ovary cells transfected with the human apolipoprotein A-I gene》.pdf

11 Biochimica et Biophysicu Acta, 1047 (1990) 11-18 Elsevier BBALJP 53491 Physical and chemical characteristics of apolipoprotein A-I-lipid complexes produced by Chinese hamster ovary cells transfected with the human apolipoprotein A-I gene T.M. Forte I, M.R. McCall I, S. Amacher ‘, R.W. Nordhausen ‘, J.L. Vigne ’ and J.B. Mallory ’ ’ Luwrenee Berkeley ~bo~a~o~, Unioers~~ of Cul~orn~o, Berkeley and ’ Cul~or~ia 3io~ech~oio~ Inc., Mountain View, CA (U.S.A.~ (Received 5 March 1990) (Revised manuscript received 12 June 1990) Key words: Apo~poprotein A-I; Chinese hamster ovary cells; Apolipoprotein A-I gene transfection; Nascent HDL; Lecithin :cholesterol acyltransferase Chinese hamster ovary cells transfected with the human apolipoprotein A-I gene linked to the human metallothionein gene promoter region secrete large quantities of apolipoprotein A-I (7.1 f 0.4% total secreted protein) in the presence of zinc. Approx. 16%of the secreted apolipoprotein A-I is complexed with lipid and can be isolated ultracentrifugally at d I 1.21 g/ml. The latter complexes are composed of discs and vesicles as judged by electron microscopy and can be further separated by cohumr chromatography into three fractions: fraction I, mostly vesicles (60-260