Opisthorchis viverrini Implications of the systematics of first intermediate hosts, Bithynia snail species in Thailand and Lao PDR》.pdfVIP

Infection, Genetics and Evolution 14 (2013) 313–319 Contents lists available at SciVerse ScienceDirect Infection, Genetics and Evolution journal homepage: www.else /loc ate/meegid Opisthorchis viverrini: Implications of the systematics of first intermediate hosts, Bithynia snail species in Thailand and Lao PDR Nadda Kiatsopit a,b, Paiboon Sithithaworn a,b,⇑, Weerachai Saijuntha c, Trevor N. Petney d, Ross H. Andrews a,b,e a Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand b Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand c Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Mahasarakham 44150, Thailand d Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany e Imperial College London, Faculty of Medicine, St. Mary’s Campus, South Wharf Street, London W2 1NY, United Kingdom a r t i c l e i n f o a b s t r a c t Article history: Bithynia snails are important hosts of the human liver fluke, Opisthorchis viverrini. Allozyme markers were Received 28 August 2012 used to examine genetic variation and the systematics of Bithynia snails from different geographical local- Received in revised form 21 December 2012 ities in Thailand and Lao PDR. Our results show that Bithynia siamensis goniomphalos contains two major Accepted 22 December 2012 evolutionary clades with at least nine morphologically similar but genetically distinct ‘‘cryptic’’ species Available online 8