Immune-regulation of the apolipoprotein A-IC-IIIA-IV gene cluster in experimental inflammation》.pdfVIP

/locate/issn Cytokine 31 (2005) 52e63 Immune-regulation of the apolipoprotein A-I/C-III/A-IV gene cluster in experimental inflammation Marıa A. Navarro, Rakel Carpintero, Sergio Acın, Jose Miguel Arbones-Mainar,´ ´ ´ Lucıa Calleja, Ricardo Carnicer, Joaquın C. Surra, Mario A. Guzman-Garcıa,´ ´ ´ ´ Nieves Gonzalez-Ramon, Marıa Iturralde, Fermın Lampreave,´ ´ ´ ´ Andres Pineiro, Jesu´ s Osada´ ˜ * ´ ´ Departamento de Bioquımica y Biologıa Molecular y Celular, Universidad de Zaragoza, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, E-50013 Zaragoza, Spain Received 16 February 2004; received in revised form 4 March 2005; accepted 23 March 2005 Abstract Apolipoprotein A-IV is a member of the apo A-I/C-III/A-IV gene cluster. In order to investigate its hypothetical coordinated regulation, an acute phase was induced in pigs by turpentine oil injection. The hepatic expression of the gene cluster as well as the plasma levels of apolipoproteins were monitored at different time periods. Furthermore, the involvement of the inflammatory mediators’ interleukins 1 and 6 and tumor necrosis factor in the regulation of this gene cluster was tested in cultured pig hepatocytes, incubated with those mediators and apo A-I/C-III/A-IV gene cluster expression at the mRNA level was measured. In response to turpentine oil-induced inflammation, a decreased he