Immunohistochemical, biochemical and electron microscopic analysis of myelin formation in the central nervous system of myelin deficient (mld) mutant mice》.pdfVIP

Developmental Brain Research, 35 (1987) 111-121 | 11 Elsevier BRD 50588 Immunohistochemical, biochemical and electron microscopic analysis of myelin formation in the central nervous system of myelin deficient (mld) mutant mice Katsuhiko Mikoshiba 1, Hideyuki Okano 1 , Yoshiro Inoue 2, Masatoshi Fujishiro 3, Ken Takamatsu 3, Franqois Lachapelle 4, Nicole Baumann 4 and Yasuzo Tsukada 3 1Division of Regulation of Macromolecular Function, Institutefor Protein Research, Osaka University, Osaka (Japan), 2 Department of Anatomy, Hokkaido University School of Medicine, Sappora (Japan), 3Department of Physiology, School of Medicine, Keio University, Tokyo (Japan) and 4Laboratoire de Nearochimie, I. N. S. E. R.M. U. 143, HOpitalde la Salpdtri~re, Paris (France) (Accepted 27 January 1987) Key words: Myelin deficient (mid) mutant mouse; Shiverer (shi) mutant mouse; Major dense line; Myelin basic protein (MBP); 2 ,3-Cyclic nucleotide 3-phosphodiesterase (CNPase); Myelin Myelin deficiency (mid) is an autosomal recessive mutation in mice and is considered to be allelic to the shiverer (shi) mutation. Mid mice are characterized by hypomyelination of the central nervous system (CNS). They show typical symptoms such as tremor, tonic convulsion and ataxic movement. Subcellular fractionation of the CNS revealed that the MBP bands were greatly decreased in the P2A (myelin) fraction and the total content of myelin basic protein (MBP) was much lower than that in the control in all parts of the CNS. Sections from mid mice were examined by immunohistochemical tests with MBP antiserum,