Hepatic translation of apolipoprotein A-I mRNA is apparently enhanced in copper-deficient rats》.pdfVIP
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Hepatic translation of apolipoprotein A-I mRNA is apparently enhanced in copper-deficient rats》.pdf
NutritionResearch, Vol. 18, No. 11.pp. 1915-1922.1998 CopyrightQ 1998 ELsevierScience Inc. Printedin the USA. All rightsreserved 0271~5317/98%19.00+.oo ELSEVIER PI1SO271-5317(98)00161-4 HEPATIC TRANSLATION OF APOLIPOPROTEIN A-I mRNA IS APPARENTLY ENHANCED IN COPPER-DEFICIENT RATS John Y.J. Wu, Scott K. Reaves, Yi Ran Wang, and Kai Y. Lei* Department of Nutritional Sciences, University of Arizona Tucson, AZ 85721 (USA) ABSTRACT Male weanling Sprague-Dawley rats were assigned to Cu-deficient (0.6 mg Ct.g diet) and Cu-adequate (6.0 mg Cu/kg diet) dietary treatments for 6 weeks. Liver post- mitochondrial cytoplasmic extracts were partitioned by centrifugation into polysomes, monosomes, and ribosomal subunits along a linear sucrose gradient. This gradient was further fractionated. The apolipoprotein (apo) A-I mRNA abundance among individual fractions and in the unfractionated cytoplasmic extract were determined by dot blot analysis. The abundance of cytoplasmic apo A-I mRNA was not different between treatments. However, Cu
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