Immunochemical characterization of two antigenic sites on human apolipoprotein A-I; localization and lipid modulation of these epitopes》.pdfVIP

160 Biochimica et Biophysics Acta 959 (1988) 160-168 Elsevier BBA 52768 Immunochemical characterization of two antigenic sites on human apolipoprotein A-I; localization and lipid modulation of these epitopes F. Pio a, H. De Loof b, N. Vu Dac a, V. Clavey a, J.C. Fruchart a and M. Rosseneu b ’ SERLIA, Department of Lipids-Lipoproteins and INSERM U 279, Institut Pasteur, Lille (France) and b Department of Clinical Biochemistry, Academisch Ziekenhuis St. Jan, Bruges (Belgium) (Received 30 September 1987) Key words: Monoclonal antibody; HDL; Dimyristoylphosphatidylcholine recombination; Apolipoprotein A-I; Competitive inhibition; Antigenic site; (Human plasma) Two monoclonal antibodies, Al7 and A30, were raised against human apolipoprotein A-I (apo A-I). They were studied by competitive inhibition of ‘25I-labeled HDL, with HDL subfractions, delipidated apo A-I, and complexes of dimyristoylphosphatidylcholine (DMPC) containing apo A-I and apo A-II. Immunoblotting located the Al7 antibody on CNBr fragment 4 of apo A-I and the A30 antibody on CNBr fragment 1. The Al7 antigenic determinant was e