Immunoaffinity fractionation of high-density lipoprotein subclasses 2 and using anti-apolipoprotein A-I and A-II immunosorbent gels》.pdfVIP

292 Biochimica et BiophysicaActa, 1002(1989)292-301 Elsevier BBA $3081 Immunoaffinity fractionation of high-density lipoprotein subclasses 2 and 3 using anti-apolipoprotein A-I and A-II immunosorbent gels Richard W. James 1, A m a n d a Proudfoot 2 and D a n i e l Pometta 1 I Lipid Laboratory, Division de Diab~toio$ie, D~partement de M~deeine 1. H~pital Cantonal, Geneva and : D~panement de Biochimie Mddicale, CMU, Geneva (Switzerland) (Received 10November 1988) Key words: HDL; ApolipoproteinA-l; ApolipoproteinA-iI; lmmunoaffinitychromatography; Monoclonalantibody; (Human) High-deuslty lipoprotein (HDL) subclasses 2 and 3 IWePared by density gradient ultracentrifngaflon have been further frartioMted by immunoaffinity chromatography using antibody affinity gels targetting the major HDL apolipoproteins, A-I and ~-II. Fractions containing A-i without A.II (ALlw / o All) and A-! with A-iI (A! w AH) were isoaated from both density ranges. Whereas there were similar concentrations of the major subfmction (HDL3(A! w All)) in both males and fentale~ rite remaining sulffraetinns were present in higher concentrations in females as compared to males, in the order HDL~ (A! w / o All) HDL2(Ai w All) HDL2(A| w / o All). The difference was most marked for HDL z (A| w / o All), where plasma concenaatious in females were almost 3-fold greater than in males. Compositional analyses indi