Hepatic siRNA delivery using recombinant human apolipoprotein A-I in mice》.pdfVIP

Biochemical and Biophysical Research Communications 378 (2009) 192–196 Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.else /loc ate/ybbrc Hepatic siRNA delivery using recombinant human apolipoprotein A-I in mice Hyeon Lee a, Soo In Kim a, Duckhyang Shin a, Yeup Yoon a, Tae Hyun Choi b, Gi-Jeong Cheon b, Meehyein Kim a,* a Virus Research Laboratory, Mogam Biotechnology Research Institute, 341 Bojeong-dong, Giheung-gu, Yongin-si, Gyeonggi-do 449-913, South Korea b Emergency Medical Team and Nuclear Medicine Laboratory, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, Seoul 139-706, South Korea a r t i c l e i n f o a b s t r a c t Article history: Apolipoprotein A-I (apo A-I), the major protein component of high density lipoprotein (HDL), plays a key Received 20 October 2008 role in reverse cholesterol transport from peripheral tissues to liver or steroidogenic organs. Class B, type Available online 17 November 2008 1 scavenger receptor (SR-BI) is abundantly expressed in these target tissues and recognizes apo A-I of HDL for selective cholesteryl ester uptake. Recently, we reported the liver-targeting potential of plasma-derived apo A-I and the efficient delivery of therapeutic small interfering RNAs (siRNA) assem- Keywords: bled with cationic liposome and apo A-I. In this study, we expressed and purified recombinant hu