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Effect of Different Energy Sources on Ochrobactrum anthropi Bacteria Chromium Reduction.pdf
30卷 6期 结 构 化 学 (JIEGOUHUAXUE) Vo1.30,No.6
2011.6 Chinese Struct. Chem. 819~826
EffectofDifferentEnergySourceson
Ochrobactrum anthropiBacteriaChromium Reduction①
MA Xiao.Yana~ PAN Xiao.Hong, CHENG YangJiana
、 UZhao—Xiann PAN Dan.mei CHEN Zhi
LIBin LIN Zhanga~ ( AN Xiongb@
(StateKeyLaboratoryofStructuralChemistry,FujianInstituteofResearchonthe
StructureofMatter,ChineseAcademyofSciences,Fuzhou,Fujian350002,China)
(KeyLabofBiopesticideandChemicalBiology,FujianAgriculutreand
ForestryUniversity,MinistryofEducation,Fuzhou,Fujian350002,China)
ABSTRACT Ochrobactrum anthropiCTS一325isolatedfrom achromium-contaminatedsite
hadbeRerresisatncetoCrpcI)inLBmediumunderaerobiccondition.Meanwhile。itwasfound
thatthereductionofCr(VI)isnotcompleteduringhteexperimentalprocess.Therefore,aseries
ofsmallmoleculeenergysourcesincludingnitrogenandcarbonsourceswereaddedintohteLB
medimu ni htebacterialsattionary phasetopromotehtechromi um reducibility.Theresult
showed htathte bacterialgrowth waspositively correlated wiht hte chromimu reduction.
SDSanalysisindicatedthattheproteinrgoupswerechangedwhenthebacteriawere
stimulatedbyhtechromimu .Additionally,itwasrevealedthatD.anthropiCTS,325could
utilizehtecheaperalternativeofsugar(sucroseresidueleachingsolution)wellofrfurther
growthandrestarthtechromium reduction,whichofferedanew methodforpracticalappli—
cations.
Keywords:Ochrobactrum anthropiCTS-325,chromium-reduction,energysources
un.renlovableandlesstoxic【,21
1 INTRoDUCTIoN .
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