大鼠胫骨标准实验性骨不连模型制作.docVIP

大鼠胫骨标准实验性骨不连模型的制作 房国军1，曲志国1，崔正宏1，刘? 英2 吉林省四平市中心医院，1骨科，2干细胞实验中心，吉林省四平市? 136000 Establishment of an experimental tibial nonunion model in rats Fang Guo-jun1, Qu Zhi-guo1, Cui Zheng-hong1, Liu Ying2 1Department of Orthopedics, Siping Central Hospital, Siping 136000, Jilin Province, China; 2Experimental Center for Stem Cells, Siping Central Hospital, Siping 136000, Jilin Province, China 摘要? 背景：建立客观标准的骨不连动物实验模型是骨不连的实验研究及探索骨不连治疗的必要条件。目的：为骨不连的实验研究建立一种客观的动物实验模型。方法：选取SD大鼠，在胫骨中段截断，用口腔科粘固粉充填器(3#或4#)灼烧骨折断端(包括骨膜)，然后用??? 0.5 mm克氏针钻入骨髓腔，固定骨折，2个月后经大体标本、病理组织学及放射学检查确定骨不连形成情况。结果与结论：大体标本观察、放射学检查及病理组织学检查均显示骨缺损区无骨性连接，为纤维瘢痕组织填充，骨端硬化。结果表明实验所建立的动物模型，具有骨不连的病理改变，符合骨不连的要求，是一种可靠而实用的实验性骨不连动物模型。 中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程 全文链接： 关键词?：?实验动物；?组织构建；?胫骨；?骨不连；?模型；?放射学；?病理学； ?? Abstract： BACKGROUND: Establishment of an objective and standard experimental animal model of bone non-union is necessary for experimental study and related treatment of non-union.OBJECTIVE: To establish an objective experimental animal model for the experimental study of non-union.METHODS: Sprague-Dawley rats were included in this study, the middle tibia was cut off, and fracture fragments (including periosteum) were seared with dental cement plugger (3# or 4#). Subsequently, bone marrow cavity was drilled using a 0.5 mm Kirschner wire, and the fractures were fixed. 2 months later, the specimens were detected by gross observation, histopathological detection and radiological examination to determine the nonunion formation.RESULTS AND CONCLUSION: The results of gross observation, histopathological detection and radiological examination showed that, no bony union was found at bone defect area, which was filled by fiber scar tissue, and bone end sclerosis was apparent. The animal models established in this study have pathological changes of bone non-union, which is in line with the requirements of bone non-union, so the model can be considered a reliable and practical animal mode