玉米杂交种先玉335和其亲本种子萌发过程中胚芽蛋白质组学分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2011, 37(9): 16891694 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2011.01689 玉米杂交种先玉335 及其亲本种子萌发过程中胚芽蛋白质组学分析 进茜宁 付志远 丁 冬 刘宗华 李卫华 汤继华* / , 450002 : 84 h (), 335 , 335 560 , PH6WC PH4CV 507 508 , 335 81%, 288 , 15 , 335 13 5 : ; (); ; Proteomic Analysis of Plumule in Seed Germination for an Elite Hybrid Pio- neer 335 and Its Parental Lines in Maize * JIN Xi-Ning, FU Zhi-Yuan, DING Dong, LIU Zong-Hua, LI Wei-Hua, and TANG Ji-Hua College of Agronomy, Henan Agricultural University / Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China Abstract: The plumule (including coleoptile) of seed cultured for 84 h was used as materials to analyze the proteomic difference between the elite hybrid Pioneer 335 and its parental lines by mean of 2-D electrophoresis method. The results showed that 560 protein spots were identified in Pioneer 335, 507 and 508 protein spots parental lines in its PH6WC and PH4CV, respectively. And in Pioneer 335, 81% protein spots exhibited nonadditive expression model. Out of them, 288 showed up-regulation with over high parent expression model. While only 15 showed down-regulation with over low parent expression model. Thus the accumulation of nonadditive expression protein might be the key factor resulting in heterosis at the tested development at stage in Pioneer 3