谷氨酸转运体在体外培养大鼠皮质星形胶质细胞机械性损伤中表达变化.pdfVIP

17 4 2008 4 　Vol.17 　No.4 　 Apr.2008 Acta Academiae Medicinae CPAF ·283 · 谷氨酸转运体在体外培养大鼠皮质星形胶质细胞机械性 损伤中的表达变化 赵志勇, 刘　芳, 彭凤翔 (武警四川总队医院, 四川乐山, 614000) 　:　【】, 。【】, Western Blotting 。【】, 12 h 24 h , (EAAT 1) (P0.05), 12 h 24 h (P0.05);Western blotting , 、 12 h 24 h (EAAT 1) (A):0.21、 0.44 0.46。 , 12 h 24 h EAAT 1 (P0.05), (P0.05)。【】EAAT 1 , 。 :;;; 【】　1008-5041(2008)04-0283-04　 【】　R745.7 　【】　A　 Glutamate transporters expression in cultured rat cortex astrocyte in vitro following me- chanical brain injury ZHAO Zhi-yong, LIU ang, PENG eng-xiang (Sichuan Provincial Corps Hospital of Chinese People s Armed Po- lice orce, Leshan 614000, China) Abstract:【Objective】To investigate the changes of gl tamate transporters expression after mechanical inj ry, and ana- lyze the possible mechanism.【Methods】To identify and q antitate astrocyte and gl tamate transporters changes in vitro sing imm nohistochemical staining flow cytometry and w estern blotting.【Results】Flow cytometry showed expression of EAAT 1 increased post-inj ry 12 h and 24 h, as compared with control (P 0.05).Western blotting show ed the absorbance (A)of 12 h and 24 h after inj ry was increased as compared with control gro p (P0.05).Two inj ry gro ps did not show obvio s differentce in expression.【Conclusions】Mechanical inj ry co ld res lt EAAT 1 expression increase in astrocyte, and EAAT 1 may have have some effect d ring brain inj ry. Key words:Gl tamate transporters;Astrocyte;Cortex;Inj ry 　　 EAAT4 EAAT5 5 , , , 。 GLAST ( , , EAAT 1)、 G LT 1 (EAAT2)、 EAAC1 (EAAT3 )、 , 。, 【】2007-11-11;【】2008-03-05 , 【】 (1976-), , , , [ 1, 2] , 。 。