心肌缺血后处理与缺血预适应的相关性研究（职称论文资料）.docVIP

心肌缺血后处理与缺血预适应的相关性研究（职称论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：心肌缺血后处理与缺血预适应的相关性研究 1 1 材料与方法 2 2 结 果 5 3 讨 论 5 文2：舒芬太尼后处理对心脏瓣膜置换术患者心肌缺血再灌注损伤的影响 6 1 资料与方法 6 2 结果 7 3 讨论 8 参考文摘引言： 9 原创性声明（模板） 10 文章致谢（模板） 10 正文 心肌缺血后处理与缺血预适应的相关性研究（职称论文资料） 文1：心肌缺血后处理与缺血预适应的相关性研究 The Research for Relatiohip between Ischemic Postconditioning and Ischemic Preconditioning Wei Xiaoxia，Xiao Chuahi，Bian Yunfei Department of Cardiology，The Second Hospital，Shanxi Medical Univeity （Taiyuan 030001） Abstract:Objective To compare the cardioprotective effects of ischemic postconditioning and ischemic preconditioning on ischemia-reperfusion（IR） injury of rats in Thirty Wistar rats were randomly divided into five groups: Sham-operated group，IR group，ischemic postconditioning group，ischemic preconditioning group，ischemic preconditioning，and postconditioning myocardial ischemia-reperfusion models of rats were cardiac treponin I （cTnI） were tested with biochemistry infarct size was measured by image analytical Compared with IR group，infarct size and the levels of cTnI were significantly less in postconditioning group，preconditioning group，preconditioning group and postconditioning were no differences between the three Ischemic postconditioning was as effective as ischemic preconditioning in reducing infarct size and serum combined protective effects did not appear to be additive，this probably meant that there was same or similar cardioprotective mechanism. Key words:myocardium；ischemia reperfusion injury；ischemic postconditioning；ischemic preconditioning 在心肌梗死、体外循环手术，器官移植等许多危重状态下，往往需要早期再灌注治疗，但是再灌注又可加重心肌的缺血损伤［1，2］，研究证实缺血预适应与缺血后处理均可显著减轻心肌缺血再灌注损伤。本实验利用大鼠在体心肌缺血再灌注模型，观察缺血后处理与缺血预适应对心肌的保护作用是否一致，两种保护措施同时合用是否有叠加效应。 1 材料与方法 药品与试剂、仪器 血浆肌酸激酶（CK）购自南京建成生物工程研究所。血浆肌钙蛋白I（cTnI）试剂盒购自美国LDI公司。Even’s blue染液及TTC 染液均购自Sigma公司。RM6240B型生理实验系统购自成都仪器厂。HX-300动物呼吸机及BL-2000图像分析系统购自成都泰盟科技有限公司。 实验动物及分组 健康Wistar大鼠30只，由山西医科大学实验动物中心提供，体重220 g～250 g，雌雄不限。随机分为5组，每组6只。假手术组：只在左冠状动脉前降支（LAD）下穿线，不结扎；缺