清开灵对缺氧体外血脑屏障紧密连接蛋白ZO1表达的影响（职称论文资料）.docVIP

清开灵对缺氧体外血脑屏障紧密连接蛋白ZO1表达的影响（职称论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：清开灵对缺氧体外血脑屏障紧密连接蛋白ZO1表达的影响 1 1 材料与方法 3 2 结 果 5 3 讨 论 6 文2：金钠多对体外缺氧致人大动脉内皮细胞分泌内皮素的影响 7 1材料和方法 8 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 清开灵对缺氧体外血脑屏障紧密连接蛋白ZO1表达的影响（职称论文资料） 文1：清开灵对缺氧体外血脑屏障紧密连接蛋白ZO1表达的影响 Effects of Qingkailing Injection on Expression of Tight Junction ZO-1 of Blood brain Barrier in Vitro Iulted by Hypoxia Zhu Haiyan，Ma Tao，Lou Lixia，et al Beijing Key Laboratory of TCM Protection and Utilization，Beijing Normal Univeity（Beijing 100875） Abstract:Objective To observe the effects of hypoxia on expression of tight junction ZO-1 of blood brain barrier（BBB） in vitro and the role of Qingkailing The BBB in vitro was established by culturing mouse cerebral microvasculature endothelial cells of model of ischemic injury was established by oxygen and sugar be deprived for 24 gene tracription and protein expression level of ZO-1 were measured by fluorescence real-time quantitative and western blot ，the expression of ZO-1 caused by different dose of Qingkailing injection was compared with control group，the expression of ZO-1 in BBB was reduced significantly after oxygen and sugar be deprived for 24 h（P）.Compared with model group，the expression of gene tracription and protein expression of ZO-1 were increased in Qingkailing injection group，and there was statistical difference（P or P）.Conclusion Qingkailing injection had cerebral protection role in ischemic stroke by improving the expression of main component protein of BBB damaged by brain ischemia. Key words:Qingkailing injection；blood brain barrier；hypoxia；ZO-1 缺血性中风一直是临床上的治疗难题，血脑屏障（blood brain barrier，BBB）的破坏既是脑缺血损伤的结果，又是进一步导致脑损伤的关键环节［1］，而BBB通透性的改变与紧密连接（tight junction，TJ）的主要成分ZO-1分布及缺失密切相关［2］。清开灵对缺血性中风急性期患者有良好的治疗作用［3，4］。探讨脑缺血后BBB 上主要功能蛋白ZO-1的改变及清开灵对其表达的影响，对脑中风初期的治疗及预后具有重要意义，同时还有助于揭示清开灵抗脑缺血性损伤的作用机制。 1 材料与方法 细胞 Balb/c小鼠脑微血管内皮细胞系细胞（中日友好医院娄晋宁教授惠赠），经抗Ⅷ因子抗体、抗CD31抗体及DiI-乙酰化低密度脂蛋白标记摄取检测，符合内皮细胞特性［5］ 主要药品及试剂 清开灵注射液（每支10 mL