几种环境雌激素和DNA相互作用检测-食品工程专业论文.docxVIP

Ab Abstract PAGE PAGE III Abstract Environmental Estrogens (EEs) is a kind of external chemical substance, which can disrupt the normal endocrine of human and animals, they can through the combined with estrogen receptors, competition the binding sites on the receptor binding, play the similar effect of estrogen and affect the cell signaling pathways. As an important carrier of organisms genetic material, DNA is one of the main targets of many small molecules, these small molecules may cause damage to the DNA molecule, and harm the growth of the organism. Therefore inord to provide theoretical basis, for further understanding the mechanism of action of environmental estrogen and DNA, the study of between the EEs and DNA is very important. In this paper, combined use of A variety of spectroscopy, chemometrics techniques, and the molecular simulation to simulate human physiological acidity (pH 7.4) condition, researched of the three kinds of environmental estrogens (diethylstilbestrol, bisphenol A, bisphenol AF) interaction with calf thymus DNA. The main contents of these investigations were summarized as follows: A brief introduction of the physiological characteristics of DNA and the main harm of environmental estrogens, introduction the basic theory and testing method about how to study the interaction of biological molecules and small molecules. Under the conditions in the human body physiological acidity (pH 7.4), with acridine orange (AO) as a fluorescence probe, using fluorescence spectrometry, circular dichroism (CD) and fourier transform infrared spectroscopy (FT–IR), and viscosity measurements, measured the interaction of environmental estrogen diethylstilbestrol (DES) with calf thymus DNA (ctDNA). Results showed that DES bound to A, T base pairs of ctDNA through intercalation, swap out the AO which before embedded into the ctDNA. The binding constant reached of 104, the thermodynamic parameter change value of enthalpy and entropy were both less than zer