电针对力竭游泳大鼠肾脏线粒体自由基代谢和线粒体功能影响.docVIP

电针对力竭游泳大鼠肾脏线粒体自由基代谢和线粒体功能影响 　　(1.河南省体工大队医务所,郑州450003;2.河南中医学院针推系)[摘　要]　目的:进一步研究电针的抗过氧化、抗衰老作用,探讨肾俞平衡阴阳、益精补肾机理。方法:选择7组大鼠做力竭游泳实验,观察力竭前后不同时间及有无电针刺激条件下大鼠肾脏线粒体自由基代谢情况和线粒体功能的变化。结果:发现力竭游泳后即刻和4小时末MDA,GSH,NADH,游离Ca2+,THG,GSH?Px均有不同特点、程度的变化;电针组大鼠游泳耐力时间明显长于未电针组(P 　　[主题词]　电针;线粒体/针灸效应;自由基/代谢;游泳 　　Effect of Electroacupuncture on Metabolism of Mitochondrial Free Radicals and Mitochondrial Function in Kidney in Rats of Full Swimming 　　Luo Lei?1,Xu Xiaojin?2(1.Infirmary of Henan Province?s Physical Training Team,Zhengzhou 450003;2.Department of Acupuncture and Massage,Henan College of TCM) 　　[Abstract]　Purpose　To study further anti?hyperoxidation and anti?ageing action of electroacupuncture and preliminarily approach to the machanism of Shenshu(BL 23) in balancing Yin and Yang,supplementing vital essence and tonifying ?kiidney. Methods　Changes of metabolism of mitochondrial free radicals and mitochondial function in kidney were investigated at different times after full swimming and electroacupuncture.Results　MDA,GSH,NADH,free Ca2+,THG,GSH?Px at 0 and 4 hours after swimmimg had changes in varying degrees;the swimming time in the electroacupuncture group was significantly longer than that in the nonelectroacupuncture group.Conclusion　Full swimming can induce a series of hyperoxidation,and production,accumalation and abnormal metabolism of a great number of free radicals in rats;Electroacupuncture at Shenshu can obviously increase the ability of anti?hyperoxidation,effectively clearing free radicals and increasing motor ability,which reveal preliminarily the mechanism of Shenshu in balancing Yin and Yang,supplementing vital essence and tonifying kidney. 　[Keywords]Electroacupuncture;Mitochondria/acup eff;Free Radicals/metab;Swimming 　　 　　越来越多的研究证实,化学性质极为活泼的自由基可引发机体过氧化反应,导致生物膜损伤、生物分子交联等一系列生理、生化紊乱[1]。人体运动实验显示,长时间有氧运动至力竭可使机体自由基增加,消除能力下降,以致自由基积累,产生疲劳[2]。关于自由基的危害,有研究表明,大鼠长时间大强度运动训练后线粒体膜流动性下降,刚性增加,这是自由基对膜脂质双分子层结构攻击所致[3]。有报道称针刺对中风、脑瘫等患者体内脂质过氧化水平具有一定的良性影响[4]。传统中医认为刺激肾俞可平衡阴阳,益精补肾,这种作用是否