电针对MCAO大鼠脑内GAP-43和突触囊泡蛋白表达影响.docVIP

电针对MCAO大鼠脑内GAP-43和突触囊泡蛋白表达影响 　　［摘要］方法：将实验大鼠分为假手术组、缺血再灌组和缺血再灌＋电针组，大脑中动脉线栓(MCAO)造成局灶性缺血90min，电针组在缺血后立即给予电针1 h，以后每天1次。在缺血再灌1，7和14天分别处死，进行免疫组织化学染色。结果：GAP-43和突触囊泡蛋白主要在缺血灶周围的皮质表达。在再灌后7和14天后电针组GAP-43和突触囊泡蛋白免疫阳性细胞数高于缺血再灌组(P＜0.05)。结论：电针能够提高GAP-43和突触囊泡蛋白在缺血灶周围皮质的表达，从而促进缺血后轴突的出芽和突触的形成，提高了缺血后神经元可塑性。 　　［主题词］电针；生长结合蛋白-43*；突触膜糖蛋白/代谢；再灌注损伤/针灸疗法Effect of Electroacupuncture on GAP-43 and Synaptophysin Expression of Ischemic Brain in the MCAO Rat 　　Chen Yinghui, Huang Xianfen (Department of Neurobiology, Shanghai Medical University, Shanghai 200032, China) 　　［Abstract］Purpose To observe the effect of electroacupuncture (EA) on growth-associated protein (GAP-43) and Synaptophysin expression of ischemic brain in the rat by immunohistochemical method. Methods The experiment was divided into three groups: sham operation group, ischemia group and ischemia plus EA group. The middle cerebral artery (MCA) was occluded for 90 min to result in the local ischemia. EA was applied for one hour immediately after cerebral ischemia. Then EA was applied every day till the rats were decapitated after reperfusion for 1,7 and 14 days, respectively. Immunohistochemical method was used. Results GAP-43 and Synaptophysin immunoreactive cells expressed mainly in the surrounding cortex of infarction. In the EA group GAP-43 and Synaptophysin immunoreactive cells after reperfusion for 7 and 14 days were significantly higher than those in the ischemia group (P＜0.05).Conclusion EA may increase expression of GAP-43 and Synaptophysin in the surrounding cortex of infarction, so EA can promote the neuronal sprouting and synaptogenesis after ischemia, and improve the neuronal plasticity. 　　［Key words］Electroacupuncture; Growth-Associated Protein*; Synaptophysin/metab; Reperfusion Injury/acup ther 　　脑缺血将导致梗死区的大量神经元死亡，从而使得一定区域内失去神经支配。但这也刺激了未损伤神经元的出芽和建立新的突触联系。这种出芽和突触再生支持??缺血损伤后神经元修复和可塑性的假说。与出芽和突触囊泡有关的蛋白的增加将伴随轴突和树突的出芽以及突触数量的改变。轴突出芽作为解剖可塑性的组成部分能够通过生长结合蛋白-43 (growth-associated protein,GAP-43)的高表达来鉴定。GAP-43是一种膜结合蛋白，它被发