ap4在结直肠癌局部淋巴结中的表达与结直肠癌复发相关性的分析word格式论文.docxVIP

优秀毕业论文 精品参考文献资料 336, Detection of AP-4 expression and clinical pathologic parameters. Criterion: In paraffin sections,AP-4 protein expression was mainly localized in the nucleus, showing brownish yellow granules. According to the proportion of all cells and staining intensity of positive cells, the expression of the AP-4 to do semi-quantitative determination.The results by two experienced pathologist assessed, get the mean. Determine the results: A: Points by the number of color cells, no positive staining in tumor cells as compared with 0 points, positive cell rate of ≤ 10% to 1 score, 11% to 50% for 2 score, 51% to 75% for the 3 score, 75% 4 score. B: points by cell color depth, no positive cells were 0 score, light yellow to 1 score, brown for 2 minutes, dark brown for 3 minutes. Number of points = A × B. 0 score as -, 1 to 4 score as + , 5 to 8 as + +, 9 to 12 as + + +. Get 280 of fresh lymph node diameter ≥ 5mm (1-41 pieces / cases with a median of 6.5 / cases) from 28 patients with resected colorectal cancer patients, by RT-PCR method to detect AP-4 in fresh colorectal cancer lymph node tissues. SPSS13.0 statistical analysis software used to analyze the expression of the AP-4 and clinical pathological factors using chi-square test, P 0.05 as statistically significant. Results 1、Immunohistochemistry:(1) in 73 patients with colorectal cancer in 594 lymph node detection in 314 AP-4 expression positive in lymph node (positive rate was 52.86%), 258 of lymph node metastasis in 242 lymph node expression of AP-4 positive (positive rate of 93.80%), 72 examine AP-4 expression positive in 336 HE staining of normal lymph node tissue (positive rate was 21.43%); (2) 47 cases of Ⅲ - Ⅳ patients with only three cases of lymph node-negative, 44 patients more than one or more lymph nodes were detected in AP-4 expression positive; 26 cases in the 0 ~ Ⅱ patients, 11 cases (42.31%) patients with lymph node-negative AP-4 expression (P 0.01), the remaining 15 cases (57.69%) patients