血清及组织中的分布.PDFVIP

158 2013, Vol.34, No.24 食品科学 ※分析检测 UPLC-Q-TOF-MS检测槲皮素代谢产物在大鼠 血清及组织中的分布 邢萌萌，朱科学，聂少平，李 娜，李文娟，杨 希，谢明勇* (南昌大学 食品科学与技术国家重点实验室，江西 南昌 330047) 摘  要：目的：采用四极杆-飞行时间串联质谱(Q-TOF-MS)检测分析大鼠口服槲皮素单体后血清和组织中的代谢 产物。方法：血清和组织样品采用Sephadex LH-20 固相萃取小柱进行分离纯化，使用Q-TOF-MS检测，以体积分数 0.1% 甲酸溶液- 乙腈为流动相梯度洗脱，电喷雾离子源负离子条件下进行扫描，鉴定大鼠给药槲皮素单体后血清及 组织中的代谢产物。结果：槲皮素在血液中主要以其与葡糖醛酸、硫酸酯结合物的形式存在，约占全部代谢物的 73.70%，一部分也以其与脂肪酸结合物的形式存在，约占26.30%；此外，槲皮素在体内分布广泛，在组织中的总 含量由高到低依次为乳腺＞子宫＞卵巢＞肾上腺＞心＞脾＞肝＞肾。结论：Q-TOF-MS可快速、有效地鉴定出SD大 鼠血清及组织中的槲皮素代谢产物，为今后进行槲皮素与人类健康效应相关的研究提供参考。 关键词：槲皮素；四极杆-飞行时间串联质谱仪；葡糖醛酸；硫酸酯；代谢物 Determination of Quercetin Metabolites in Rats Serum and Tissues by UPLC-Q-TOF-MS XING Meng-meng，ZHU Ke-xue ，NIE Shao-ping，LI Na，LI Wen-juan ，YANG Xi ，XIE Ming-yong* (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China) Abstract ：Obj ective: To determine quercetin metabolites in serum and tissues of rats after intragastric administration of quercetin aglycone by quadrupole-time of flight-mass spectrometry (Q-TOF-MS). Methods: The pre-processed serum and tissue samples were separated and purified by Sephadex LH-20 solid-phase column. The collected aliquots were analyzed by Q-TOF-MS in the negative ion mode using gradient elution with a mobile phase composed of 0.1% formic acid (A) and acetonitrile (B) to identify the metabolites of quercetin. Results: The Q-TOF-MS analysis showed that quercetin was primarily metabolized to glucuronides and sulfates (73.70%) as well as esters (26.30%). Moreover, quercetin and quercetin metabolites were widely distributed in rat tissues, especially in the female reproductive organs in SD rats. Its total concentration in tissues was as fol