远隔缺血后处理对小鼠前脑缺血再灌注损伤模型海马.docVIP

精品论文 参考文献 远隔缺血后处理对小鼠前脑缺血再灌注损伤模型海马 郗东锋 毛慧斌 　　（山西阳泉煤业集团总医院麻醉科 山西阳泉 045000） 　　【摘要】目的 初步探讨肢体缺血后处理对脑缺血/再灌注损伤的影响，验证小鼠前脑缺血再灌注损伤模型开展相关研究的可行性。方法 30只小鼠随机分为3组：假手术组（A组），缺血60 min组（B组），缺血60 min ＋后处理组（C组）。用双颈总动脉阻断法建立小鼠前脑缺血/再灌注损伤模型，以可复性阻断小鼠双侧股动脉作为干预措施。实验小鼠再灌注24 h后取脑，记数海马CA1区的存活细胞数、变性细胞率。结果 海马CA1区细胞存活数，A组gt; C组gt; B组（P lt; 0.01）；细胞变性率，B组、C组都大于A组（P lt; 0.05），但B组和 C组比较差异无统计学意义（Pgt; 0.05）。结论 肢体缺血后处理可减轻脑缺血/再灌注损伤；小鼠前脑缺血再灌注损伤模型可作为开展相关研究的平台。 　　【关键词】 远隔缺血后处理 缺血/再灌注损伤 海马CA1区 　　【中图分类号】R965 【文献标识码】A 【文章编号】2095-1752（2014）02-0091-02 　　 　　Morphological effects of remote ischemic postconditioning on hippocampus gyrus CA1 area in mice with forebrain ischemia-reperfusion XI Dong-feng, MAO Hui-bin (Dept of Anaesthesiology, Yangquan Coalmine Group General Hospital, Shanxi，Yangquan 045000) 　　【Abstract】 Objective To investigate effects of remote ischemic postconditioning on forebrain ischemia-reperfusion injury in mice and establish a animal model for related experiments. Methods Thirty mice were randomly divided into 3 groups: sham operation group (A), ischemia for 60 min group (B), RIPC group (C). The mouse model of forebrain ischemia-reperfusion was established by blocking bilateral common carotid arteries. RIPC group received 3 cycles of 5 min reperfusion followed by 5 min ischemia in bilateral femoral arteries at the beginning of cerebral reperfusion. After 24 h reperfusion, Comparison of the number of viable cell and denatured cell rates in hippocampus gyrus CA1 area between 3 groups. Results Comparison of the number of viable cell , group A gt; group Cgt; group B（P lt; 0.01）. Compared with group A, denatured cell rates of group B and C are higher(P lt; 0.05). In addition, there is no significant difference between group B and C (P gt; 0.05). Conclusion RIPC could protect brain against forebrain ischemia-reperfusion induced injury. The mouse model of 60 min ischemia and 24 h reperfusion is suitable for experiments concer