细胞生物化学实验课件Exp 6-Cell Biology Experiment I- Yingyu Cui.pdfVIP

Cell Biology Experiment Course for MBBS Experiment I Ordinary optical Microscopy Ying-Yu Cui Division of cell biology Department of Regenerative medicine Tongji University School of medicine E-mail: yycui@tongji.edu.cn Overview • Objective • Principle • Procedure • Results and analysis • Notes • Thought questions Objectives • To obtain knowledge objectives ①building blocks of microscope ; ② working principles of microscope; ③usage skills of microscope; ④ Biological drawing skills. • To obtain ability objectives ①logic thinking; ②working in pairs; ③communication skills • To obtain moral objectives ①polite; ②team-working spirit; ③self care their things well 2014/9/3 Key and difficult points for learning • Key points: ①microscope structures ②working principle of microscope ③methods to using microscope correctly ④power or magnification of an ordinary optical microscope • Difficult points: ①understand the working principle of microscope ②find a clear image of some object rapidly under microscope ③draw a cell amplified under microscope 2014/9/3 Principles • Any optical lens made of transparent material or glass with curved surface can make object amplifying and imaging. • Modern optical microscope usually adopts two-stage amplification, accomplished by objective lens and eye-lens, respectively. First-stage amplification makes its inverted real image, which then will be further amplified second-stage by eye lens into inverted virtual image, or the image caught by human eyes. • An objective lens with very short focal length (often a few mm) is used to form a magnified real image of the object, then of the eyepiece, usually a c