丹参酮iia缓解大鼠自体移植静脉桥内膜增生的研究.docVIP

精品论文 参考文献 丹参酮IIA缓解大鼠自体移植静脉桥内膜增生的研究 霍天明 覃秀秀 梁洪华 韦武扬 覃家锦(通讯作者) 　　（广西医科大学 广西南宁 530021） 　　【摘要】 目的：探讨丹参酮IIA（TA）对大鼠自体移植静脉移植物内膜增生的影响。方法：28只雄性SD大鼠随机分为实验组和对照组，建立颈外静脉移植颈总动脉模型。实验组予口服TA 13.3mg /（kg?d），对照组同方法予同剂量生理盐水。术后28d采集移植静脉桥，计算新生内膜厚度。人大隐静脉平滑肌细胞同样分为实验组（TA干预）和对照组（PBS干预）。MTT法观察细胞增殖情况，Transwell小室观察细胞迁移情况。结果：相比对照组移植静脉桥，实验组厚度显著减少[(58.13plusmn;5.40)mu;m vs（39.22plusmn;6.32)mu;m,P＜0.05]。实验组相比对照组，人大隐静脉平滑肌细胞增殖与迁移率均受到抑制（P＜0.05）。结论：TA可抑制静脉桥内膜增生，应与抑制静脉平滑肌细胞增殖与迁移有关。 　　【关键词】丹参酮IIA；静脉桥；内膜增生；再狭窄；平滑肌细胞 　　【中图分类号】R654 【文献标识码】A 【文章编号】1007-8231（2015）24-0010-02 　　Effect of tanshinone IIA on intimal hyperplasia in autogenous vein grafts of rats Huuo Tianming,Qin Xiuxiu,Liang Honghua,Wei Wuyang,Qin Jiajin. Guangxi Medical University,Nanning 530021,China 　　【Abstract】Objective To study the effects of tanshinone IIA(TA) on intimal hyperplasia in autogenous vein grafts of rats.Methods After jugular vein-to-artery bypass grafting surgery,28 male SD rats were randomly divided into the treatment group by orally administered of TA 13.3mg/(kg?d), and the control group by saline.The vein grafts were harvested after 28 days.HE staining was performed for histological changes of grafted wein intimal membranes. Human saphenous vein smooth muscle cells were treated with TA or PBS. Cell proliferation was assayed by MTT and migration was observed by Transwell chamber. Results Intimal thickness in treatment group was statistically lower when compared to control group[(58.13plusmn;5.40)mu;m vs(39.22plusmn;6.32)mu;m,P＜0.05] after 28 days. Cell proliferation and migration was markedly inhibited(P＜0.01). Conclusion TA can obviously inhibit hyperplasia of intimal thickness after venous transplantation, which may be related to the inhibition of smooth muscle cell proliferation and migration. 　　【Key words】Tanshinone IIA;Vein graft;Intimal hyperplasia;Restenosis;Smooth muscle cell 　　冠状动脉旁路移植术是现今治疗终末期冠脉严重病变的重要手段。大隐静脉是最常用的移植血管。但术后5～10年通畅率约50%[1]，使研究如何提高静脉桥血管远期通畅率成为热点。 　　丹参是一种传统的中