ghrelin 基因arg51glu 多态性与2 型糖尿病易感性的相关性研究.docVIP

精品论文 参考文献 Ghrelin 基因Arg51Glu 多态性与2 型糖尿病易感性的相关性研究 张莹丽 刘静 尹虹 郭瑾通讯作者（甘肃省兰州市第一人民医院 730050） 　　【摘要】 目的：探讨生长素(Ghrelin)基因多态性在甘肃省甘南藏族自治州藏族人群中的分布及其与2型糖尿病的关系。方法：应用PCR-RFLP技术检测104例2型糖尿病和107名健康对照者Ghrelin的基因型,分别以氧化酶法、放免法、比色法测定他们的空腹血糖、空腹血胰岛素、糖化血红蛋白水平。结果：Ghrelin基因型GG、GA频率在2型糖尿病组和对照组分别为0.885、0.115和0.963、0.037;等位基因G、A频率在2型糖尿病组和对照组分别为0.942、0.058和0.981、0.019。其基因型频率和等位基因频率在2型糖尿病组和对照组比较差异均有统计学意义(均Plt;0.05)。基因型频率的相对风险分析发现, GA基因型患2型糖尿病的风险是GG基因型3.359倍(OR=3.359，95%CI：1.047-10.778)。结论：Ghrelin Arg51Glu基因多态性与藏族人群2型糖尿病的发病有相关性,A等位基因可能是2型糖尿病的易感基因。 　　【关键词】 糖尿病,2型; Ghrelin;；藏族；多态现象(遗传学)【中图分类号】 R2 【文献标号】 A 【文章编号】 2095-7165(2015)14-018-02Association of Arg51Glu polymorphism of the ghrelin gene with susceptibility to type 2diabetes mellitus in Gansu Zang population Zhang Ying Li1, Liu Jing2, Yin Hong1 Guo Jin11 The First Peopleprime;s Hospital of Lan Zhou(2 Department of endocrinology, Gan Su ProvinceCorresponding author : Zhang Ying Li, Email: zhangyingli80@126.com)[Abstract] bjective　To investigate the ghrelin gene polymorphism in Chinese Zang population and its association with type 2 diabetes mellitus (DM).Methods　Genotype of ghrelin was determined in 104 patients with type 2 DM and 107 healthy controls by PCR- RFLP. Plasma glucose、insulin andHbA1c levels were measured by biochemical technique . Results　 ghrelin genotype frequencies of GG、GA were 0.885、0.115 in type 2 DM groupand 0.963、0.037 in control group respectively , and allele frequencies of G、A were 0.942、0.058 in type 2 DM group and 0.981、0.019 in control grouprespectively . There were significant difference in frequencies of allele and genotype in ghrelin Arg51Glu polymorphism between type 2 DM group andcontrol group (both Plt;0.05).The relative risk of GA genotype suffered from type 2 DM was3.359 times of the GG genotype (OR=3.359，95%CI：1.047-10.778). Conclusion　 ghrelin Arg51Glu polymorphism is associated with type 2 DM and Aallele appears to be a risk factor for type 2 DM in Zang population .[Keywords] Diabetes mellitus , type 2 ; ghr