小麦抗赤霉病和蚜虫转基因20种载体构建与转化示例.pdfVIP

Journal of Integrative Agriculture Advanced Online Publication: 2014 Doi: 10.1016/S2095-3119(13)60735-2 Transgenic Expression of a Functional Fragment of Harpin Protein Hpa1 in Wheat Represses English Grain Aphid Infestation XU Man-yu, ZHOU Ting, ZHAO Yan-ying, LI Jia-bao, XU Heng, DONG Han-song and ZHANG Chun-ling National Ministry of Education Key Laboratory of Integrated Management of Crop Diseases and Pests/ College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.China Abstract The harpin protein Hpa1 produced by the rice bacterial blight pathogen promotes plant growth and induces plant resistance to pathogens and insect pests. The region of 10-42 residues (Hpa110-42) in the Hpa1 sequence is critical as the isolated Hpa110-42 fragment is 1.3-7.5-fold more effective than the full length in inducing plant growth and resistance. Here we report that transgenic expression of Hpa110-42 in wheat induces resistance to English grain aphid, a dominant species of wheat aphids. Hpa110-42-induced resistance is effective to inhibit the aphid behavior in plant preference at the initial colonization stage and repress aphid performances in the reproduction, nymph growth, and instar development on transgenic plants. The resistance characters are correlated with enhanced expression of defense-regulatory genes (EIN2, PP2-A , and GLS10) and consistent with induced expression of defense response genes (Hel, PDF1.2, PR-1b, and PR-2b). As a result, aphid infestations are alleviated in transgenic plants. The level of Hpa110-42-induced resistance in regard to repression of aphid infestations is equivalent to the effect of chemical control provided by an insecticide. These re