高产红曲黄色素菌株的选育.PDFVIP

微生物学通报 DEC 20, 2008, 35(12): 1909~1914 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 高产红曲黄色素菌株的选育 周 波 王菊芳 吴振强 梁世中* ( 510641) 摘 要: 利用紫外、硫酸二乙酯、氯化锂和亚硝基胍复合诱变的方法, 选育到一株高产黄色素的 红曲霉突变株 MYM2 。经过稳定性实验证明, 诱变得到的菌株稳定性较好, 液态发酵试验黄色素 色价达到100 U/mL 以上, 黄色素色调达到3.5 左右。此黄色素在300 nm~600 nm 波长之间只有一 个在410 nm 附近的最大吸收峰, 在pH 3~8 之间稳定性较好。当pH 小于3 时, 红曲黄色素不稳定, 黄色素溶液变混浊, 放置后有沉淀产生。 关键词: 红曲霉, 突变株, 黄色素, 色价, 色调 Selection of Monascus with High Yellow Pigment Production * ZHOU Bo WANG Ju-Fang WU Zhen-Qiang LIANG Shi-Zhong (School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641) Abstract: The Monascus mutant with high yield of yellow pigment was obtained by using conventional relevant mutation techniques, e.g., treating with physical mutagens(such as UV light) and chemical sub- stances (such as N-methyl-N-nitro-N-nitrosoguanidine). The yellow pigment was scanned from 300 nm to 600 nm with UV spectrometer, the maximal absorption was determined at 410 nm. The growth characteristic of Monascus mutant is stable, the yellow pigment value and colour hue in liquid fermentation can reach 100 U/mL and 3.5 respectively. The yellow pigment is stable from pH 3 to pH 8, but the precipitation appeared as the pH of the pigment solution lower than 3. Keywords: Monascus , Mutant, Yellow pigment, Pigment value, Colour hue , Monascorubramine , 60%, , ,