pAKT、HIF-1α在胃癌中表达及及血管生成关系.docVIP

pAKT、HIF-1α在胃癌中表达及及血管生成关系 　 作者：顾晓萌　卢雪峰　王慧　付金栋　季锐　姜大磊　雒燕 【摘要】 　　目的: 研究磷酸化丝氨酸/苏氨酸蛋白激酶(pAKT)、缺氧诱导因1α(HIF-1α)在胃癌中的表达及AKT/HIF-1α途径与胃癌的发生和血管生成的相关性。方法: 免疫组化法检测64例胃癌及26例癌旁正常组织中pAKT蛋白和HIF-1α的表达，以CD34标记血管内皮,计数微血管密度(MVD)。结果: 64例胃癌组织中pAKT呈阳性表达48例(75%)，HIF-1α阳性表达42例(65.6%)，均显著高于癌旁组织(χ2分别为26.936、15.950，Plt;0.05)；pAKT、HIF-1α表达与肿瘤的TNM分期、浸润深度、淋巴结转移、远处转移显著相关(Plt;0.05)。胃癌及癌旁组织MVD分别为43.5±12.7、26.2±12.3， Plt;0.05(t=5.614)； pAKT、HIF-1α均阳性患者MVD显著多于pAKT、HIF-1α均阴性者(t=3. 947,Plt;0.05),pAKT与HIF-1α的表达呈正相关(Pearson r=0.583，Plt;0.05)。结论：pAKT蛋白过表达于胃癌组织，AKT蛋白的磷酸化可增强胃癌细胞的侵袭能力，促进胃癌的转移,又可以通过调节HIF-1α的表达促进胃癌新生血管的生成。同时检测胃癌组织中pAKT、HIF-1α和MVD的表达状态，对于胃癌的早期诊断和治疗有重要的指导价值。 【关键词】 缺氧诱导因子1α· 磷酸化丝氨酸/ 苏氨酸蛋白激酶·胃肿瘤·微血管密度 　　Relationship between pAKT, HIF-1α expression and angiogenesis in gastric cancer 【ABSTRACT】 Objectives: To investigate the expression of pAKT and HIF-1α and to analyze the relationship among AKT/HIF-1α pathway, the angiogenesis and carcinogenesis in human gastric carcinoma. Methods: The expression of pAKT and HIF-1αin 64 gastric carcinomas and 26 normal tissues were evaluated by immunohistochemistry. CD34 immunostaining was used to measure the microvascular density (MVD). Results: pAKT-positive expression was detected in 75% (48/64) of the tumors, HIF-1α-positive expression was 65.6%. The expression levels of pAKT and HIF-1α proteins in cancerous tissues were higher than those in normal tissues (Plt;0.05). The expression levels of pAKT or HIF-1α were significantly associated with TNM stage, invasion depth, lymph node metastasis or distant metastases (Plt;0.05). MVD of gastric cancer (43.5±12.7) was much higher than that of normal tissues (26.2±12.3), MVD was much higher in tumor with pAKT(+) /HIF-1α(+) than those in tumor with pAKT(-)/HIF-1α(-) (t=3.947, Plt;0.05), pAKT expression was significantly correlated with HIF-1α(r=0.583, Plt;0.05). Conclusion: pAKT is distinctively over expressed in gastric tissues and its phosphorylation may promote the invasion and metastases of gastric cancer. Addition