P16及PTEN蛋白在食管鳞癌组织中表达及临床意义.docVIP

P16及PTEN蛋白在食管鳞癌组织中表达及临床意义 　【摘要】 目的 探讨抑癌基因p16、PTEN蛋白的表达与食管鳞癌的关系,为食管鳞癌预后判断提供依据。方法 采用免疫组织化学法检测67例食管鳞癌标本中p16、PTEN蛋白的表达。结果 p16蛋白阳性表达率为47.8% ,PTEN 蛋白阳性表达率为58.2%。p16、PTEN 蛋白表达与肿瘤的浸润深度、淋巴结转移情况显著相关，且p16与PTEN蛋白的表达具有正相关（r=0.782,Plt;0.005）。结论 p16、PTEN蛋白联合检测可提高对食管鳞癌预后预测的敏感性。 【关键词】 食管鳞癌 p16 PTEN 免疫组织化学 EXPRESSION AND SIGNIFICANCE OF TUMOR Abstract Objective To investigate the relationship between the exp- ression of tumor suppressor gene p16 and PTEN protein and the biological behaviors of esophageal squamous cell carcinoma tissue. Methods　The expression of p16 and PTEN proteins in 67 esophageal squamous cell cancer specimens was investigated with immunohistochemical technique using p16 and PTEN antibodies. The relationship between the expression of p16 and PTEN proteins and the clinicopathological parameters of esophageal squamous cell cancer was analyzed. Results　The frequency of expression of p16 and PTEN proteins was 47.8% and 58.2% in esophageal squamous cell cancer, respectively. The expression of p16 and PTEN proteins was positively correlated with the imbibitions and metastasis of esophageal squamous cell cancer (P lt;0.05 respectively). Moreover, there was a positive correlation between the p16 and PTEN protein expression in colorectal cancer (r =0.782, P lt;0.005). Conclusion　Expression of tumor suppressor gene p16 and PTEN might be helpful in predicting the biological behavior and prognosis of esophageal squamous cell cancer. Key words Esophageal squamous cell cancer P16 PTEN Immunohistochemistry 在食管癌变过程中, 多种癌基因的激活和抑癌基因的失活发挥着极其重要的作用［１］。p16 基因又名多瘤抑制基因(multiple tumour suppressor1 ,MTS1),位于染色体9q21 ,是直接参与细胞周期调控的新型抑癌基因。PTEN 基因(phosphatase and tensin homolog deleted from chromosome 10 ) 是目前发现的第一个具有磷酸酶活性的抑癌基因。本文应用SP免疫组化技术, 检测了抑癌基因p16、PTEN在67例食管鳞癌组织中的表达情况。 1 材料与方法 1.1 材料 67例食管癌标本取自青海大学附属医院2006年11月至2008年3月手术切除标本，其中男性48例，女性19例，年龄在40～72，平均(58.04±4.76)岁。所有病例就诊前均未经任何放疗、化疗。67例均经病理组织学确诊为鳞状细胞癌,其中Ⅰ级27例, Ⅱ级21例, Ⅲ级19例; 20例(浅层组)肿瘤浸润黏膜层、黏膜下层或浅肌层, 47例(深层组)肿瘤浸润深肌层或外膜层;有淋巴结转移者27例,无淋巴结