MGMT及Survivin在乳腺癌中表达及其临床意义.docVIP

MGMT及Survivin在乳腺癌中表达及其临床意义 　 作者：王发亮，薄爱华，芦广萍，侯金超，吴荣薇 【摘要】 目的：研究MGMT和Survivin在乳腺癌中的表达及其临床意义。方法：福尔马林固定，石蜡包埋乳腺癌和腺瘤标本，采用SABC免疫组织化学方法检测MGMT和Survivin在这些组织中的表达。结果：MGMT和Survivin在乳腺癌和乳腺腺瘤中的表达有显著性差异。MGMT在乳腺癌中的表达与患者的年龄、淋巴结转移有关，而Survivin仅与淋巴结转移有关。另外，MGMT和Survivin之间具有相关性（r=0.48,Plt;0.01）。结论：MGMT和Survivin的异常表达与乳腺癌的淋巴结转移有关；MGMT和Survivin可以作为判断乳腺癌发生和预后的重要指标；检测它们的表达可以指导临床上化疗方案的制定。 【关键词】 O6甲基鸟嘌呤DNA甲基转移酶；生存素；乳腺肿癌 Breast cancer is a common female malignant tumor.In recent years,the incidence of breast cancer is increasing in China while operation is the main therapeutic method nowadays.Because the genesis mechanism of breast cancer has not been clarified,and the effective pathway for predication was deficient,thus most patients with breast cancer were diagnosed at late stage,and they have missed the best opportunity for an operation.The curative rate will be increased if the pathogenesis is illuminated.It is usually considered that abnormal accumulation of gene mutation and the strong effects of Antiapoptosis proteins play important roles in the progression of tumor genesis.Recently an investigation has shown that MGMT and Antiapoptosis protein Survivin play important roles in the progression of breast cancer.In this study,to investigate the effects of MGMT and Survivin in the formation and progression of breast cancer,the expression of them in breast adenoma and breast cancer was detected. 1 MATERIALS AND METHODS 1.1 Clinical Data All cases were selected from the First Affiliated Hospital of Hebei North University,including 121 cases with breast cancer and 22 cases with breast adenoma.Among the 121 cases with breast cancer,57 cases were left,others were right,the that of patients with carcinoma was 47.8(29～77 years),and the that of patients with adenoma was 35.7(22～51 years).All the patients were not treated before specimens were taken.Among them,67 cases had lymph node metastasis,and 54 cases hadnt.All tissues were fixed with 10%formalin and embedded with paraffin,and two se