combining next-generation sequencing strategies for rapid molecular resource development from an invasive aphid species, aphis glycines结合下一代测序策略快速分子入侵蚜虫物种的资源开发,aphis甘氨酸.pdfVIP

Combining Next-Generation Sequencing Strategies for Rapid Molecular Resource Development from an Invasive Aphid Species, Aphis glycines 1 1 1 1,2 1 2 Xiaodong Bai , Wei Zhang , Lucia Orantes , Tae-Hwan Jun , Omprakash Mittapalli , M. A. Rouf Mian , Andrew P. Michel1* 1 Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, Columbus, Ohio, United States of America, 2 United States Department of Agriculture - Agricultural Research Services and Department of Horticulture and Crop Sciences, The Ohio State University, Columbus, Ohio, United States of America Abstract Background: Aphids are one of the most important insect taxa in terms of ecology, evolutionary biology, genetics and genomics, and interactions with endosymbionts. Additionally, many aphids are serious pest species of agricultural and horticultural plants. Recent genetic and genomic research has expanded molecular resources for many aphid species, including the whole genome sequencing of the pea aphid, Acrythosiphon pisum. However, the invasive soybean aphid, Aphis glycines, lacks in any significant molecular resources. Methodology/Principal Findings: Two next-generation sequencing technologies (Roche-454 and Illumina GA-II) were used in a combined approach to develop both transcriptomic and genomic resources, including expressed genes and molecular markers. Over 278 million bp were sequenced among the two methods, resulting in 19,293 transcripts and 56,688 genomic sequences. From this data set, 635 SNPs and 1,382 microsatellite markers were identified. For each sequencing method, different soybean aphid biotypes were used which revealed potential