有哪些信誉好的足球投注网站biofilm development on caenorhabditis elegans by yersinia is facilitated by quorum sensing-dependent repression of type iii secretion生物膜发展线虫通过鼠疫是促进群体sensing-dependent镇压iii型分泌.pdfVIP
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biofilm development on caenorhabditis elegans by yersinia is facilitated by quorum sensing-dependent repression of type iii secretion生物膜发展线虫通过鼠疫是促进群体sensing-dependent镇压iii型分泌
Biofilm Development on Caenorhabditis elegans by Yersinia Is Facilitated by Quorum Sensing-Dependent Repression of Type III Secretion 1 1 2 1 1 Steve Atkinson , Robert J. Goldstone , George W. P. Joshua , Chien-Yi Chang , Hannah L. Patrick , ´ 1 2 1 Miguel Camara , Brendan W. Wren , Paul Williams * 1 School of Molecular Medical Sciences, Centre for Biomolecular Science, University of Nottingham, Nottingham, United Kingdom, 2 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom Abstract Yersinia pseudotuberculosis forms biofilms on Caenorhabditis elegans which block nematode feeding. This genetically amenable host-pathogen model has important implications for biofilm development on living, motile surfaces. Here we show that Y. pseudotuberculosis biofilm development on C. elegans is governed by N-acylhomoserine lactone (AHL)- mediated quorum sensing (QS) since (i) AHLs are produced in nematode associated biofilms and (ii) Y. pseudotuberculosis strains expressing an AHL-degrading enzyme or in which the AHL synthase (ypsI and ytbI) or response regulator (ypsR and ytbR) genes have been mutated, are attenuated. Although biofilm formation is also attenuated in Y. pseudotuberculosis strains carrying mutations in the QS-controlled motility regulator genes, flhDC and fliA, and the flagellin export gene, flhA, flagella are not required since fliC mutants form normal biofilms. However, in contrast to the parent and fliC mutant, Yop virulon proteins are up-regulated in flhDC, fliA and flhA mutants in a temperature and calc
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