combining the physical adsorption approach and the covalent attachment method to prepare a bifunctional bioreactor结合物理吸附方法和共价连接的方法来准备一个双官能团的生物反应器.pdfVIP

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combining the physical adsorption approach and the covalent attachment method to prepare a bifunctional bioreactor结合物理吸附方法和共价连接的方法来准备一个双官能团的生物反应器.pdf

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combining the physical adsorption approach and the covalent attachment method to prepare a bifunctional bioreactor结合物理吸附方法和共价连接的方法来准备一个双官能团的生物反应器

Int. J. Mol. Sci. 2012, 13, 11443-11454; doi:10.3390/ijms130911443 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor Mengxing Dong †, Zhuofu Wu †, Ming Lu, Zhi Wang and Zhengqiang Li * Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China; E-Mails: dong.meng.xing@163.com (M.D.); wuzf06@ (Z.W.); luming@ (M.L.); wangzhi@ (Z.W.) † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: lzq@; Tel./Fax: +86-431-8515-5201. Received: 30 July 2012; in revised form: 28 August 2012 / Accepted: 5 September 2012 / Published: 12 September 2012 Abstract: Aminopropyl-functionalized SBA-15 mesoporous silica was used as a support to adsorb myoglobin. Then, in order to avoid the leakage of adsorbed myoglobin, lysozyme was covalently tethered to the internal and external surface of the mesoporous silica with glutaraldehyde as the coupling agent. The property of amino-functionalized mesoporous silica was characterized by N adsorption-desorption and thermogravimetric (TG) analysis. 2 The feature of the silica-based matrix before and after myoglobin adsorption was identified by fourier transform infr