biophysical assessment of single cell cytotoxicity diesel exhaust particle-treated human aortic endothelial cells生物物理评估单个细胞的细胞毒性柴油机尾气particle-treated人类主动脉内皮细胞.pdfVIP

Biophysical Assessment of Single Cell Cytotoxicity: Diesel Exhaust Particle-Treated Human Aortic Endothelial Cells 1¤ 2 2 1 3 3 Yangzhe Wu , Tian Yu , Timothy A. Gilbertson , Anhong Zhou *, Hao Xu , Kytai Truong Nguyen 1 Department of Biological Engineering, Utah State University, Logan, Utah, United States of America, 2 Department of Biology, Utah State University, Logan, Utah, United States of America, 3 Department of Bioengineering, The University of Texas at Arlington, Arlington, Texas, United States of America Abstract Exposure to diesel exhaust particles (DEPs), a major source of traffic-related air pollution, has become a serious health concern due to its adverse influences on human health including cardiovascular and respiratory disorders. To elucidate the relationship between biophysical properties (cell topography, cytoskeleton organizations, and cell mechanics) and functions of endothelial cells exposed to DEPs, atomic force microscope (AFM) was applied to analyze the toxic effects of DEPs on a model cell line from human aortic endothelial cells (HAECs). Fluorescence microscopy and flow cytometry were also applied to further explore DEP-induced cytotoxicity in HAECs. Results revealed that DEPs could negatively impair cell viability and alter membrane nanostructures and cytoskeleton components in a dosage- and a time-dependent manner; and analyses suggested that DEPs-induced hyperpolarization in HAECs appeared in a time-dependent manner, implying DEP treatment would lead to vasodilation, which could be supported by down-regulation of cell biophysical properties (e.g., cell elasticity). These findings are consistent with the conclusion that DEP exposure triggers important biochemical and biophysi