yy1-binding sites provide central switch functions in the parp-1 gene expression networkyy1-binding网站提供中央开关功能的parp-1基因表达网络.pdfVIP

下载本文档

3
0
约10.53万字
约 16页
2017-09-12 发布于上海
举报
版权申诉

yy1-binding sites provide central switch functions in the parp-1 gene expression networkyy1-binding网站提供中央开关功能的parp-1基因表达网络.pdf

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

yy1-binding sites provide central switch functions in the parp-1 gene expression networkyy1-binding网站提供中央开关功能的parp-1基因表达网络

YY1-Binding Sites Provide Central Switch Functions in the PARP-1 Gene Expression Network 1,2 1,3 ´4 1,5 ´ 1,4 Martina Doetsch , Angela Gluch , Goran Poznanovic , Juergen Bode , Melita Vidakovic * 1 Helmholtz Centre for Infection Research/Epigenetic Regulation, Braunschweig, Germany, 2 Department of Biochemistry and Molecular Cell Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria, 3 BIOBASE GmbH, Wolfenbuettel, Germany, 4 Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Belgrade, Serbia, 5 Hannover Medical School (MHH), Experimental Hematology, Hannover, Germany Abstract Evidence is presented for the involvement of the interplay between transcription factor Yin Yang 1 (YY1) and poly(ADP- ribose) polymerase-1 (PARP-1) in the regulation of mouse PARP-1 gene (muPARP-1) promoter activity. We identified potential YY1 binding motifs (BM) at seven positions in the muPARP-1 core-promoter ( 2574/+200). Binding of YY1 was observed by the electrophoretic supershift assay using anti-YY1 antibody and linearized or supercoiled forms of plasmids bearing the core promoter, as well as with 30 bp oligonucleotide probes containing the individual YY1 binding motifs and four muPARP-1 promoter fragments. We detected YY1 binding to BM1 (2587/ 2558), BM4 (2348/2319) and a very prominent association with BM7 (+86/+ 115). Inspection of BM7 reveals overlap of the muPARP-1 translation start site with the Kozak sequence and YY1 and PARP-1 recognition sites. Site-directed mutagenesis of the YY1 and PARP-1 core motifs e