who needs microtubules myogenic reorganization of mtoc, golgi complex and er exit sites persists despite lack of normal microtubule tracks谁需要微管mtoc肌原性的重组,高尔基氏复合体和er退出网站持续尽管缺乏正常的微管跟踪.pdfVIP

Who Needs Microtubules? Myogenic Reorganization of MTOC, Golgi Complex and ER Exit Sites Persists Despite Lack of Normal Microtubule Tracks 1 1 2 1¤ 1 1,3 Kristien J. M. Zaal *, Ericka Reid , Kambiz Mousavi , Tan Zhang , Amisha Mehta , Elisabeth Bugnard , Vittorio Sartorelli2, Evelyn Ralston1 1 Light Imaging Section, Office of Science and Technology, National Institute of Arthritis, Musculoskeletal, and Skin Disease, National Institutes of Health, Bethesda, Maryland, United States of America, 2 Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, Maryland, United States of America, 3 Institut Curie, Centre Universitaire, Orsay, France Abstract A wave of structural reorganization involving centrosomes, microtubules, Golgi complex and ER exit sites takes place early during skeletal muscle differentiation and completely remodels the secretory pathway. The mechanism of these changes and their functional implications are still poorly understood, in large part because all changes occur seemingly simultaneously. In an effort to uncouple the reorganizations, we have used taxol, nocodazole, and the specific GSK3-b inhibitor DW12, to disrupt the dynamic microtubule network of differentiating cultures of the mouse skeletal muscle cell line C2. Despite strong effects on microtubules, cell shape and cell fusion, none of the treatments prevented early differentiation. Redistribution of centrosomal proteins, conditional on differentiation, was in fact increased by taxol and nocoda