whole cell imprinting in sol-gel thin films for bacterial recognition in liquids macromolecular fingerprinting完整的细胞印迹溶胶-凝胶薄膜的细菌在液体高分子指纹识别.pdfVIP

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whole cell imprinting in sol-gel thin films for bacterial recognition in liquids macromolecular fingerprinting完整的细胞印迹溶胶-凝胶薄膜的细菌在液体高分子指纹识别.pdf

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whole cell imprinting in sol-gel thin films for bacterial recognition in liquids macromolecular fingerprinting完整的细胞印迹溶胶-凝胶薄膜的细菌在液体高分子指纹识别

Int. J. Mol. Sci. 2010, 11, 1236-1252; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Whole Cell Imprinting in Sol-Gel Thin Films for Bacterial Recognition in Liquids: Macromolecular Fingerprinting Tally Cohen, Jeanna Starosvetsky, Uta Cheruti and Robert Armon * Faculty of Civil Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion, Haifa 32000, Israel; E-Mails: tallyco@tx.technion.ac.il (T.C.); starjean@tx.technion.ac.il (J.S.); uta@tx.technion.ac.il (U.C.) * Author to whom correspondence should be addressed; E-Mail: cvrrobi@tx.technion.ac.il; Tel.: +972-4-829-2-77; Fax: +972-4-829-3309. Received: 28 January 2010; in revised form: 26 February 2010 / Accepted: 22 March 2010 / Published: 24 March 2010 Abstract: Thin films of organically modified silica (ORMOSILS) produced by a sol-gel method were imprinted with whole cells of a variety of microorganisms in order to develop an easy and specific probe to concentrate and specifically identify these microorganisms in liquids (e.g., water). Microorganisms with various morphology and outer surface components were imprinted into thin sol-gel films. Adsorption of target microorganism onto imprinted films was facilitated by these macromolecular fingerprints as revealed by various microscopical examinations (SEM, AFM, HSEM and CLSM). The imprinted films