whole-genome synthesis and characterization of viable s13-like bacteriophages全基因组的合成和表征可行s13-like噬菌体.pdfVIP

Whole-Genome Synthesis and Characterization of Viable S13-Like Bacteriophages 1,2. 1. 1,4. 1 1 1 Yuchen Liu , Yonghua Han , Weiren Huang , Yonggang Duan , Lisha Mou , Zhimao Jiang , 1 1 1 1 1 1 1 Pingping Fa , Jun Xie , Ruiying Diao , Yuanbin Chen , Yiwang Ye , Ruilin Yang , Jing Chen , 1 1 1 1 1,3,4 Xiaojuan Sun , Zesong Li , Aifa Tang , Yaoting Gui *, Zhiming Cai * 1 Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU- HKUST Medical Center, Shenzhen, China, 2 Shantou University Medical College, Shantou, China, 3 The Institute of Urogenital Diseases, Shenzhen University, Shenzhen, China, 4 Shenzhen Second People’s Hospital, Shenzhen, China Abstract Background: Unprecedented progresses in high-throughput DNA sequencing and de novo gene synthesis technologies have allowed us to create living organisms in the absence of natural template. Methodology/Principal Findings: The sequence of wild-type S13 phage genome was downloaded from GenBank. Two synonymous mutations were introduced into wt-S13 genome to generate m1-S13 genome. Another mutant, m2-S13 genome, was obtained by engineering two nonsynonymous mutations in the capsid protein coding region of wt-S13 genome. A chimeric phage genome was designed by replacing the F capsid protein open reading frame (ORF) from phage S13 with the F capsid protein ORF from p