whole-tree water use efficiency is decreased by ambient ozone and not affected by o3-induced stomatal sluggishness树形水分利用效率是减少环境臭氧和不受o3-induced气孔迟缓.pdfVIP

Whole-Tree Water Use Efficiency Is Decreased by Ambient Ozone and Not Affected by O3-Induced Stomatal Sluggishness 1 1 2 Yasutomo Hoshika , Kenji Omasa , Elena Paoletti * 1 Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan, 2 Institute of Plant Protection, National Research Council, Sesto Fiorentino, Florence, Italy Abstract Steady-state and dynamic gas exchange responses to ozone visible injury were investigated in an ozone-sensitive poplar clone under field conditions. The results were translated into whole tree water loss and carbon assimilation by comparing trees exposed to ambient ozone and trees treated with the ozone-protectant ethylenediurea (EDU). Steady-state stomatal conductance and photosynthesis linearly decreased with increasing ozone visible injury. Dynamic responses simulated by severing of a leaf revealed that stomatal sluggishness increased until a threshold of 5% injury and was then fairly constant. Sluggishness resulted from longer time to respond to the closing signal and slower rate of closing. Changes in photosynthesis were driven by the dynamics of stomata. Whole-tree carbon assimilation and water loss were lower in trees exposed to ambient O3 than in trees protected by EDU, both under steady-state and dynamic conditions. Although stomatal sluggishness is expected to increase water loss, lower stomatal conductance and premature leaf shedding of injured leaves aggravated O3 effects on whole tree carbon gain, while compensating for water loss. On average, WUE of trees exposed to ambient ozone was 2–4% lower than that of EDU-protected control trees in September and 6–8% lower in October. Citation: Hoshika Y, Omasa K, Paoletti E (2012) Whole-Tree Water Use Effic