半枝莲提取物诱导人肝癌SMMC-7721细胞凋亡及其对凋亡相关蛋白表.docVIP

半枝莲提取物诱导人肝癌SMMC-7721细胞凋亡及其对凋亡相关蛋白表 　 作者：韦鹏涯 浦洪琴 韦星 宾晓芸 李朝敢 【摘要】 　　目的探讨半枝莲提取物(Scutellaria barbata D. Don extract, SBE)对人肝癌SMMC-7721细胞凋亡及凋亡相关蛋白Bcl-2，Survivin和Caspase-3表达的影响。方法体外培养SMMC-7721细胞，以2.5，5和10 mg·L-1SBE处理48 h，并以2.5 mg·L-1顺铂（DDP）作为阳性对照。用MTT法检测细胞抑制率，流式细胞仪检测细胞凋亡率，二步法免疫组化检测Bcl-2，Survivin和Caspase-3蛋白表达。结果 与正常对照组相比，SBE各浓度组细胞抑制率显著升高（P﹤0.001），细胞凋亡率明显上升（P﹤0.01），Caspase-3蛋白表达显著上升（Plt;0.01或Plt;0.05），Bcl-2和Survivin蛋白表达明显下降（Plt;0.01或Plt;0.05）。结论 SBE具有诱导肝癌SMMC-7721细胞凋亡的作用，其分子机制可能与上调Caspase-3蛋白表达以及下调Bcl-2和Survivin蛋白表达有关。 【关键词】 半枝莲 SMMC-7721细胞 细胞凋亡 Bcl-2; Survivin Caspase-3 　　Abstract：ObjectiveTo explore the apoptosis-inducing effect of Scutellaria barbata D. Don extract（SBE）and the expression of apoptosis associated proteins Bcl-2, Survivin and Caspase-3 on human liver cancer SMMC-7721 cells. MethodsSMMC-7721 cells were treated with 2.5, 5 and 10 mg·L-1 SBE for 48h, and the cells were treated with 2.5mg·L-1 DDP as positive control. The inhibitory rat was evaluated by MTT assay. The apoptotic rate was determined by flow cytometry. The expression of Bcl-2, Survivin and Caspase-3 proteins were detected by two-step immunohistochemical staining.ResultsCompared with control group, the inhibitory rate was increased obviously(Plt;0.001), the apoptotic rate was increased markedly(Plt;0.01), the expression of Caspase-3 protein was increased significantly(Plt;0.01 or Plt;0.05), while Bcl-2 and Survivin proteins were decreased markedly (Plt;0.01 or Plt;0.05) in SBE groups. ConclusionSBE can induce apoptosis of liver cancer SMMC-7721 cells. The molecular mechanism might be related to up-regulating the expression of Caspase-3 and down-regulating the expression of Bcl-2 and Survivin apoptosis related proteins. 　　Key words：Scutellaria barbata D. Don; SMMC-7721 cells; Apoptosis 　　半枝莲Scutellaria barbata D. Don，SB属于唇形科植物，临床上常用于肝癌、肺癌、胃癌和鼻咽癌等多种癌症防治，但具体的抗癌机制尚不明确。研究发现，半枝莲提取物（SBE）能诱导白血病K562细胞凋亡，其作用机制可能与Caspase3的表达升高和活化有关［1］；SBE可诱导人肝癌QGY-7701细胞凋亡，并能上调Bax和下调Bcl-2的表达［2］；SBE还通过上调凋亡相关基因蛋白Fas表达，促进人肝癌He