千里光抗金黄色葡萄球菌作用机制血清药理学探究.docVIP

千里光抗金黄色葡萄球菌作用机制血清药理学探究 　 作者：张文平　张文书　王小丽　张瑞其　黄真 【摘要】 目的应用血清药理学方法研究中药千里光抗金黄色葡萄球菌的作用及其机制。方法从千里光中分离出黄酮类化合物，然后通过给小鼠灌胃制备千里光水浸液的含药血清和黄酮类化合物的含药血清。用扫描电镜观察各含药血清对金黄色葡萄球菌超微结构的影响，用同位素前体参入试验观察各含药血清对金黄色葡萄球菌生物合成的影响。同时设立生理盐水血清作为阴性对照。结果与阴性对照血清相比，两种含药血清作用后的金黄色葡萄球菌，其形态和超微结构均发生明显的变化，菌体塌陷，细菌融合成团，呈现肿胀模糊等变化；其DNA ，RNA 蛋白质和肽聚糖合成也均受到明显的抑制。结论千里光的抗金黄色葡萄球菌作用机制可能是通过抑制细菌的DNA，RNA，蛋白质和肽聚糖的合成有关，其作用的有效成分可能是黄酮类化合物。 【关键词】 千里光；金黄色葡萄球菌；扫描电镜；同位素参入；药物作用； 血清药理学 　　Abstract：ObjectiveTo explore the antibacterial mechanism of Senecio scandens Buch-Ham(SSB) on Staphylococcus aureus by serum pharmacological method．MethodsFlavonoid compound was isolated from SSB, and then the serum contained water decoct agent of SSB or flavonoid compound were prepared with mice. By scanning electronic microscope (SEM), the effects of drug-containing serum on the ultrastructure of S. aureus were observed. By incorporation experiment of isotope-labeled precursor, the effects of drug containing serum on the biosynthesis of S. aureus were observed as well. Meanwhile, the serum contained normal saline was set as negative control.ResultsCompared with negative control serum, the morphology and ultrastructure of both drug containing serum on S. aureus showed obvious changes :the cell collapsed, melt together, blurred and swollen, ect. In addition, the synthesis of DNA, RNA, protein and peptidoglycan was inhibited.ConclusionThe antibacterial mechanism of SSB on S. aureus may be involved with the inhibition of DNA, RNA, protein and peptidoglycan synthesis, its effective ingredients may be flavonoid compound. 　　Key words：Senecio scandens Buch-Ham; Staphylococcus aureus; Scanning electronic microscope; Isotope-incorporation; Drug action; Serum pharmacology 　　千里光Senecio scandens Buch-Ham系菊科千里光属植物，我国南方各省均有分布。该药性凉味苦，具有清热解毒、消炎凉血、明目止痛、去腐生肌之功效，民间广泛用于治疗各种炎症和皮肤病。其化学成分主要有黄酮及其苷类、鞣质、生物碱等。现代药理实验表明［1，2］千里光及其含药血清对金黄色葡萄球菌等多种细菌均有较强的抗菌作用，黄酮类化合物可能是千里光的主要抗菌有效成分。为了进一步研究千里光的抗菌机制，本研究采用血清药理学方法，以常用的金黄色葡萄球菌为研究对象，结合电镜技术、同位素参入技术等观察了千里光及其黄酮类化合物对受试菌超微结构以及DNA、RNA、蛋白质和肽