免疫抑制剂姜黄素逆转胰岛素抵抗探究.docVIP

免疫抑制剂姜黄素逆转胰岛素抵抗探究 　【摘要】 【目的】 为探讨姜黄素(curcumin)对胰岛素抵抗的治疗作用及其机制。【方法】 利用胰岛素抵抗的小鼠模型，观察姜黄素对小鼠胰岛素抵抗的水平、转录因子I-?资B?琢、循环细胞因子的变化。【结果】 ①姜黄素能抑制TNF?琢刺激Fao细胞NF-?资B激活；②姜黄素治疗后小鼠的空腹血糖水平没有明显下降，但空腹胰岛素的水平下降约30％，葡萄糖耐量曲线的水平也明显下降；③循环中TNF?琢［(34.5±3.4）pg/mL vs （31.3±1.1）pg/mL］水平没有明显改变，而IL-1?茁［（33.9±3.2）pg/mL vs （15.6±1.1） pg/mL］ 和IL-6 ［(72.1±9.8）pg/mL vs （47.2±5.3） pg/mL］水平明显被抑制；④胰岛素刺激的IRS-1酪氨酸的磷酸化水平明显升高,　而胰岛素受体的磷酸化水平没有明显改变。与IRS-1酪氨酸的磷酸化水平平行，P85的磷酸化水平也明显升高，AKT的磷酸化的水平也明显升高。【结论】 姜黄素能提高小鼠胰岛素敏感性，有治疗改善肥胖诱导的胰岛素抵抗的作用。 【关键词】 姜黄素； NF-?资B； 胰岛素； 抵抗) Abstract： 【Objective】 To investigate inhibitory effect of curcumin and its mechanism on insulin resistance. 【Methods】 Curcumin co-operated high fat diets were tested for the effect of curcumin on obesity induced insulin resistance in rodent model. Transcription factor I and circulating cytokines were also detected after curcumin treatment. 【Results】 （1） Curcumin inhibited TNF?琢-stimulated NF-?资B activation in Fao cells. （2）The fasting plasma glucose level was not obviously decreased after the treatment of curcumin, but the level of fasting insulin decreased by about 30%. Furthermore, the level of glucose tolerance curve dropped significantly. （3） The level of TNF?琢［(34.5±3.4)pg/mL vs (31.3±1.1) pg/mL] in circulation was not obviously changed, but the level of IL-1?茁 [(33.9±3.2) pg/mL vs (15.6±1.1) pg/mL] and IL-6 [(72.1±9.8) pg/mL vs (47.2±5.3) pg/mL] was suppressed dramatically. （4） IRS-1 tyrosine phosphorylation levels stimulated by insulin obviously increased, but the phosphorylation level of insulin receptor did not change. Being parallel with tyrosine phosphorylation levels of IRS-1, the phosphorylation levels of P85 and AKT increased accordingly. Significantly improved insulin sensitivity was observed in groups with curcumin. 【Conclusion】 Curcumin inhibits NF-?资B dependent inflammatory pathway and reverses obesity-induced insulin resistance. Key words: curcumin, NF-?资B, insulin, resistance 姜黄（Curcuma longa L．）是常用中药，临床上用于活血、行气、通经、止痛。姜黄素（curcumin）是一