健脾活血方对2型糖尿病大鼠肝细胞膜胰岛素受体影响.docVIP

健脾活血方对2型糖尿病大鼠肝细胞膜胰岛素受体影响 　【摘要】 　　目的探讨健脾活血方对2型糖尿病胰岛素抵抗(Insulin Resistance,IR)大鼠肝细胞膜胰岛素受体（Insulin Receptor,InsR）mRNA表达的改善作用及机理。方法取Wistar大鼠60只，雌雄各半，采用链脲佐菌素(Streptozotocin,STZ)加高脂饮食造成2型糖尿病胰岛素抵抗大鼠模型。设空白组、模型组、健脾活血方低剂量组、健脾活血方高剂量组和罗格列酮组，以RT-PCR法检测大鼠肝细胞膜InsR mRNA的表达。结果健脾活血方高剂量组大鼠肝脏InsR mRNA的表达与罗格列酮组相当，与模型组比有显著性差异（Plt;0.01）。结论健脾活血方能够上调大鼠肝细胞膜InsR mRNA的表达，从而改善胰岛素抵抗。 【关键词】 健脾活血方 2型糖尿病大鼠 肝细胞膜InsR 　　Abstract：ObjectiveTo explore the effect of Strengthening Spleen and Activating Blood Prescription on expressions of insulin receptor gene in the liver membrane of Type 2 Diabetes Mellitus rats and its mechanism.Methods60 Wistar rats ,half male and half female, were randomly devided into five groups: normal control group,model group, Strengthening Spleen and Activating Blood Prescription lowdose group and highdose group, and Rosiglitazone group. The model of insulin resistance of type 2 diabetebes mellitus was established by STZ injection and high-fat diet. Expression of insulin receptor gene in liver membrane was detected.ResultsThe expressions of liver insulin receptor gene in Strengthening Spleen and Activating Blood Prescription high dose group was the same with Rosiglitazone group. There were significant differences compared with model group (Plt;0.01). ConclusionStrengthening Spleen and Activating Blood Prescription can up-regulate the expressions of liver insulin receptor gene in type 2 diabetic rats, and improve insulin resistance. 　　Key words：Strengthening Spleen and Activating Blood Prescription； Type 2 Diabetes Mellitus rats； Liver membrane insulin receptor； Experimental research 目前普遍认为，IR与Ins分泌缺陷是2型糖尿病的发病基础，其中IR是贯穿于2型糖尿病发生、发展的全过程的重要因素，IR被认为是基因与环境间相互作用的结果［1～3］，已经证实有不同种族的许多基因与2型糖尿病的发生有关联，这些后选基因包括：胰岛素基因、InsR基因等［4，5］ 。它们通过影响胰岛素信号传递和葡萄糖利用等机制在IR的发生发展中起作用［6］。本研究将从分子水平探讨健脾活血方对肝细胞膜InsR mRNA表达的改善作用和机理。 　　1 材料与仪器 　　1.1 动物Wistar大鼠60只，雌雄各半，体重220～240 g，清洁级，由黑龙江中医药大学实验动物中心提供。 　　1.2 药物健脾活血方由人参、黄芪、三七、丹参、生地等组成。饮片购自哈尔滨世一堂制药厂。将以上中药按配方煎成200%的药液(即每毫升药液含2 g原生药材煎出物)，冷却后密封，4℃保存备用；罗格列酮：太极涪陵制药有限公司，批