anti-plasmodium activity of angiotensin ii and related synthetic peptides合成肽anti-plasmodium血管紧张素ⅱ和相关活动.pdfVIP

Anti-Plasmodium Activity of Angiotensin II and Related Synthetic Peptides 1 2 ´ 3 4 Ceres Maciel , Vani Xavier de Oliveira Junior , Marcos Antonio Fazio , Rafael Nacif-Pimenta , Antonio 3 4 1 Miranda , Paulo F. P. Pimenta , Margareth Lara Capurro * ˜ ˜ 1 Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil, 2 Natural and Human Science Center, Federal University of ABC, ´ ˜ ˜ ´ Santo Andre, Brazil, 3 Department of Biophysics, Federal University of Sao Paulo, Sao Paulo, Brazil, 4 Laboratory of Medical Entomology, Rene Rachou Institute of Research, Oswaldo Cruz Foundation–FIOCRUZ, Belo Horizonte, Brazil Abstract Plasmodium species are the causative agents of malaria, the most devastating insect-borne parasite of human populations. Finding and developing new drugs for malaria treatment and prevention is the goal of much research. Angiotensins I and II (ang I and ang II) and six synthetic related peptides designated Vaniceres 1-6 (VC1-VC6) were assayed in vivo and in vitro for their effects on the development of the avian parasite, Plasmodium gallinaceum. Ang II and VC5 injected into the thoraces of the insects reduced mean intensities of infection in the mosquito salivary glands by 88% and 76%, respectively. Although the mechanism(s) of action is not completely understood, we have demonstrated that these peptides disrupt selectively the P.gallinaceum cell membr