analysis of intestinal injuries induced by ricin in vitro using spr technology and ms identification分析蓖麻毒素引起的肠道损伤体外利用spr技术和识别女士.pdfVIP

Int. J. Mol. Sci. 2009, 10, 2431-2439; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification Linna Liu 1,2, Hongwei Gao 1,*, Jiping Li 1, Ying Dong 1, Ning Liu 1, Jiayu Wan 1, Wensen Liu 1, 1 1 Yucheng Sun and Ming Xu 1 Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China; E-Mails: liulinna7@126.com (L.L.); lijiping66@126.com (J.-P.L.); lws0901@126.com (W.-S.L.); dongying0918@126.com (Y.D.); sunyuchengsyc@126.com (Y.-C.S.); wanjiayu@126.com (J.-Y.W.); xumingjlu@126.com (M.X.) 2 Department of Theoretic Veterinary Medicine, Colledge of Animal Science and Veterinary, Jilin University, Changchun 130062, China * Author to whom correspondence should be addressed; E-Mail: gaohongwei6116@; Tel. +86 431 Fax: +86 431 Received: 27 April 2009; in revised form: 14 May 2009 / Accepted: 15 May 2009 / Published: 22 May 2009 Abstract: The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an