antioxidative properties of crude polysaccharides from inonotus obliquus抗氧化性质的粗多糖纤孔菌属obliquus.pdfVIP

Int. J. Mol. Sci. 2012, 13, 9194-9206; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus Haibo Mu 1,2, Amin Zhang 1, Wuxia Zhang 1, Guoting Cui 1, Shunchun Wang 2,* and Jinyou Duan 1,* 1 College of Science, Northwest AF University, Yangling, Shaanxi 712100, China; E-Mails: mhb1025@ (H.M.); wmyzyqyybfk@126.com (A.Z.); wuxia200758@163.com (W.Z.); moohybo@ (G.C.) 2 Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China * Authors to whom correspondence should be addressed; E-Mails: shunchunwang@126.com (S.W.); jduan@ (J.D.); Tel.: +86- 21(S.W.); +86-29(J.D.); Fax: +86-21(S.W.); +86-29(J.D.). Received: 22 May 2012; in revised form: 4 July 2012 / Accepted: 16 July 2012 / Published: 23 July 2012 Abstract: The mushroom Inonotus obliquus has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA) were isolated from I. obliquus , and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively after deproteination and depigmentation. Their contents, such as neutral carbohydrate, uronic acid and protein, were measured. Their antioxidant properties