antimicrobial and efflux pump inhibitory activity of caffeoylquinic acids from artemisia absinthium against gram-positive pathogenic bacteria抗菌素和射流泵抑制活动从艾caffeoylquinic酸苦艾对抗革兰氏阳性致病菌.pdfVIP

Antimicrobial and Efflux Pump Inhibitory Activity of Caffeoylquinic Acids from Artemisia absinthium against Gram-Positive Pathogenic Bacteria Yiannis C. Fiamegos1, Panagiotis L. Kastritis2, Vassiliki Exarchou3, Haley Han4, Alexandre M. J. J. Bonvin2, Jacques Vervoort5, Kim Lewis6, Michael R. Hamblin4,7,8, George P. Tegos4,7*¤ 1 Laboratory of Analytical Chemistry, University of Ioannina, Ioannina, Greece, 2 Bijvoet Center for Biomolecular Research, Science Faculty, Utrecht University, Utrecht, The Netherlands, 3 NMR Center, University of Ioannina, Ioannina, Greece, 4 Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America, 5 Wageningen NMR Center, Laboratory of Biophysics, Wageningen, The Netherlands, 6 Department of Biology and Antimicrobial Drug Discovery Center, Northeastern University, Boston, Massachusetts, United States of America, 7 Department of Dermatology, Harvard Medical School, Boston, Massachusetts, United States of America, 8 Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts, United States of America Abstract Background: Traditional antibiotics are increasingly suffering from the emergence of multidrug resistance amongst pathogenic bacteria leading to a range of novel approaches to control microbial infections being investigated as potential alternative treatments. One plausible antimicrobial alternative could be the combination of conventional antimicrobial agents/antibiotics with small molecules which block multidrug efflux systems known as efflux pump inhibitors. Bioassay- driven purification and structural determination of compounds from plant sources have yielded a number of pump inhibitors which acted