anti-hiv-1 activity of a new scorpion venom peptide derivative kn2-7anti-hiv-1活动新的蝎毒肽kn2-7导数.pdfVIP

Anti-HIV-1 Activity of a New Scorpion Venom Peptide Derivative Kn2-7 1 1 2 2 2 2 2 Yaoqing Chen , Luyang Cao , Maohua Zhong , Yan Zhang , Chen Han , Qiaoli Li , Jingyi Yang , 2 2 2 2 2 2 2 2 1 Dihan Zhou , Wei Shi , Benxia He , Fang Liu , Jie Yu , Ying Sun , Yuan Cao , Yaoming Li , Wenxin Li , 1 1 1,2 Deying Guo , Zhijian Cao *, Huimin Yan * 1 State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China, 2 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, People’s Republic of China Abstract For over 30 years, HIV/AIDS has wreaked havoc in the world. In the absence of an effective vaccine for HIV, development of new anti-HIV agents is urgently needed. We previously identified the antiviral activities of the scorpion-venom-peptide- derived mucroporin-M1 for three RNA viruses (measles viruses, SARS-CoV, and H5N1). In this investigation, a panel of scorpion venom peptides and their derivatives were designed and chosen for assessment of their anti-HIV activities. A new scorpion venom peptide derivative Kn2-7 was identified as the most potent anti-HIV-1 peptide by screening assays with an EC50 value of 2.76 mg/ml (1.65 mM) and showed low cytotoxicity to host cells with a selective index (SI) of 13.93. Kn2-7 could inhibit all members of a standard reference panel of HIV-1 subtype B pseudotyped virus (PV) with CCR5-tropic and CXCR4- tropic NL4-3 PV strain. Furthermore, it also inhibited a CXCR4-tropic rep