anti-inflammatory activities of inotilone from phellinus linteus through the inhibition of mmp-9, nf-κb, and mapk activation in vitro and in vivo抗炎活动的inotilone phellinus linteus通过抑制mmp-9 nf-κb,激活mapk在体外和体内.pdfVIP

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anti-inflammatory activities of inotilone from phellinus linteus through the inhibition of mmp-9, nf-κb, and mapk activation in vitro and in vivo抗炎活动的inotilone phellinus linteus通过抑制mmp-9 nf-κb,激活mapk在体外和体内.pdf

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anti-inflammatory activities of inotilone from phellinus linteus through the inhibition of mmp-9, nf-κb, and mapk activation in vitro and in vivo抗炎活动的inotilone phellinus linteus通过抑制mmp-9 nf-κb,激活mapk在体外和体内

Anti-Inflammatory Activities of Inotilone from Phellinus linteus through the Inhibition of MMP-9, NF-kB, and MAPK Activation In Vitro and In Vivo 1 2 3 Guan-Jhong Huang *, Shyh-Shyun Huang , Jeng-Shyan Deng * 1 School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung, Taiwan, 2 Department of Pharmacy, College of Pharmacy, China Medical University, Taichung, Taiwan, 3 Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan Abstract Inotilone was isolated from Phellinus linteus. The anti-inflammatory effects of inotilone were studied by using lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells and l-carrageenan (Carr)-induced hind mouse paw edema model. Inotilone was tested for its ability to reduce nitric oxide (NO) production, and the inducible nitric oxide synthase (iNOS) expression. Inotilone was tested in the inhibitor of mitogen-activated protein kinase (MAPK) [extracellular signal-regulated protein kinase (ERK), c-Jun NH2-terminal kinase (JNK), p38], and nuclear factor-kB (NF-kB), matrix- metalloproteinase (MMP)-9 protein expressions in LPS-stimulated RAW264.7 cells. When RAW264.7 macrophages were treated with inotilone together with LPS, a significant concentration-dependent inhibition of NO production was detected. Western blotting revealed that inotilone blocked the protein expression of iNOS, NF-kB, and MMP-9 in LPS-stimulated RAW264.7 macrophages, significantly. Inotilone also inhibited LPS-induced ERK, JNK, and p38 phosphorylation. In in vivo tests, inotilone decreased the paw edema at the 4th and the 5th h after Carr administration, and it