anti-inflammatory cytokines predominate in acute human plasmodium knowlesi infections抗炎细胞因子在急性人类疟原虫诺氏疟原虫感染占主导地位.pdfVIP

Anti-Inflammatory Cytokines Predominate in Acute Human Plasmodium knowlesi Infections 1,2 2 1 1 1 Janet Cox-Singh *, Balbir Singh , Cyrus Daneshvar , Timothy Planche , John Parker-Williams , Sanjeev Krishna1,2 1 Division of Clinical Sciences, Infection and Immunity Research Centre, St George’s University of London, London, United Kingdom, 2 Malaria Research Centre, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kuching, Sarawak, Malaysia Abstract Plasmodium knowlesi has entered the human population of Southeast Asia. Naturally acquired knowlesi malaria is newly described with relatively little available data, including data on the host response to infection. Therefore pre-treatment cytokine and chemokine profiles were determined for 94 P. knowlesi, and for comparison, 20, P. vivax and 22 P. falciparum, patients recruited in Malaysian Borneo. Nine, five and one patient with P. knowlesi, P. falciparum and P. vivax respectively had complicated malaria as defined by World Health Organisation. Patients with uncomplicated P. knowlesi had lower levels of the pro-inflammatory cytokines IL-8 and TNFa than those with complicated disease (both p,0.05, Dunn’s post test, DPT). The anti-inflammatory cytokines IL-1ra and IL-10 were detected in all patients in the study. IL-1ra, the most abundant cytokine measured, correlated with parasitaemia in P. knowlesi (r = 0.47, p = ,0.0001), P. vivax (r = 0.61, p = 0.0042) and P. s s falciparum (r = 0.57,p = 0.0054) malaria. IL-10 correlated with parasitaemia in both P. knowlesi (r = 0.54, p = ,0.0001) and P.