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an ultra-high discrimination y chromosome short tandem repeat multiplex dna typing system一个超高歧视y染色体短串联重复序列dna多路输入系统
An Ultra-High Discrimination Y Chromosome Short
Tandem Repeat Multiplex DNA Typing System
Erin K. Hanson1,3, Jack Ballantyne1,2,3*
1 Graduate Program in Biomolecular Science, University of Central Florida, Orlando, Florida, United States of America, 2 Department of Chemistry,
University of Central Florida, Orlando, Florida, United States of America, 3 National Center for Forensic Science, Orlando, Florida, United States of
America
In forensic casework, Y chromosome short tandem repeat markers (Y-STRs) are often used to identify a male donor DNA profile
in the presence of excess quantities of female DNA, such as is found in many sexual assault investigations. Commercially
available Y-STR multiplexes incorporating 12–17 loci are currently used in forensic casework (Promega’s PowerPlexH Y and
Applied Biosystems’ AmpFlSTRH YfilerH). Despite the robustness of these commercial multiplex Y-STR systems and the ability
to discriminate two male individuals in most cases, the coincidence match probabilities between unrelated males are modest
compared with the standard set of autosomal STR markers. Hence there is still a need to develop new multiplex systems to
supplement these for those cases where additional discriminatory power is desired or where there is a coincidental Y-STR
match between potential male participants. Over 400 Y-STR loci have been identified on the Y chromosome. While these have
the potential to increase the discrimination potential afforded by the commercially available kits, many have not been well
characterized. In the present work, 91 loci were tested for their relative ability to increase the discrimination potential of the
commonly used ‘core’ Y-STR loci. The result of this extensive evaluation was the development of an ultra high discrimination
(UHD) multiplex DNA typing system that allows for the robust co-amplification of 14 non-core Y-STR loci. Population studies
with a mixed Afric
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