annotation of the transcriptome from taenia pisiformis and its comparative analysis with three taeniidae species注释的转录组绦虫pisiformis及其比较分析三个taeniidae物种.pdfVIP

Annotation of the Transcriptome from Taenia pisiformis and Its Comparative Analysis with Three Taeniidae Species 1 1 1 1 1 1 1 1 Deying Yang , Yan Fu , Xuhang Wu , Yue Xie , Huaming Nie , Lin Chen , Xiang Nong , Xiaobin Gu , 1 2 1 1 1 1 Shuxian Wang , Xuerong Peng , Ning Yan , Runhui Zhang , Wanpeng Zheng , Guangyou Yang * 1 Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, China, 2 Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya’an, China Abstract Background: Taenia pisiformis is one of the most common intestinal tapeworms and can cause infections in canines. Adult T. pisiformis (canines as definitive hosts) and Cysticercus pisiformis (rabbits as intermediate hosts) cause significant health problems to the host and considerable socio-economic losses as a consequence. No complete genomic data regarding T. pisiformis are currently available in public databases. RNA-seq provides an effective approach to analyze the eukaryotic transcriptome to generate large functional gene datasets that can be used for further studies. Methodology/Principal Findings: In this study, 2.67 million sequencing clean reads and 72,957 unigenes were generated using the RNA-seq technique. Based on a sequence similarity search with known proteins, a total of 26,012 unigenes (no redundancy) were identified after quality control procedures via the alignment of four databases. Overall, 15,920 unigenes were mapped to 203 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Through analyzing the glycolysis/ g